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Appl Environ Microbiol, May 1998, p. 1721-1724, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Improved Isolation of Vibrio vulnificus from Seawater and Sediment with Cellobiose-Colistin Agar

Lise Høi,1,* Inger Dalsgaard,2 and Anders Dalsgaard1

Department of Veterinary Microbiology1 and Danish Institute for Fisheries Research,2 The Royal Veterinary and Agricultural University, 1870 Frederiksberg C, Denmark

Received 17 November 1997/Accepted 2 March 1998

An improved selective medium, cellobiose-colistin (CC) agar, gave a significantly higher (P < 0.05) isolation rate of Vibrio vulnificus from water and sediment samples than did modified cellobiose-polymyxin B-colistin (mCPC) agar. In a total of 446 alkaline peptone water preenrichments amended with polymyxin B, V. vulnificus was isolated from 154 preenrichments (35%) with mCPC agar and from 179 preenrichments (40%) with CC agar. CC agar gave a higher plating efficiency of V. vulnificus cells than did cellobiose-polymyxin B-colistin (CPC) agar, mCPC agar, or thiosulfate-citrate-bile salts-sucrose (TCBS) agar; the only significant difference was observed with TCBS agar, which gave much lower plating efficiencies than the other selective media. Determination of MICs demonstrated that the concentrations of colistin and polymyxin B in CPC agar inhibit growth of a proportion of V. vulnificus strains.

* Corresponding author. Mailing address: The Royal Veterinary and Agricultural University, Department of Veterinary Microbiology, Bulowsvej 13, 1870 Frederiksberg C, Denmark. Phone: 4535282704. Fax: 4535282711. E-mail: lise.hoei{at}vetmi.kvl.dk.

Appl Environ Microbiol, May 1998, p. 1721-1724, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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