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Appl Environ Microbiol, May 1998, p. 1725-1730, Vol. 64, No. 5
Observatoire Océanologique, Centre
National de la recherche Scientifique, Institut National des
Sciences de l'Univers et Université Pierre et Marie Curie,
F-66651 Banyuls-sur-Mer Cedex, France
Received 29 October 1997/Accepted 9 March 1998
Seven blue nucleic acid dyes from Molecular Probes Inc. (SYTO-9,
SYTO-11, SYTO-13, SYTO-16, SYTO-BC, SYBR-I and SYBR-II) were compared
with the DAPI (4',6-diamidino-2-phenylindole) method for flow
cytometric enumeration of live and fixed bacteria in aquatic systems.
It was shown that SYBR-II and SYTO-9 are the most appropriate dyes for
bacterial enumeration in nonsaline waters and can be applied to both
live and dead bacteria. The fluorescence signal/noise ratio was
improved when SYTO-9 was used to stain living bacteria in nonsaline
waters. Inversely, SYBR-II is more appropriate than SYTO dyes for
bacterial enumeration of unfixed and fixed seawater samples.
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Comparison of Blue Nucleic Acid Dyes for Flow
Cytometric Enumeration of Bacteria in Aquatic Systems
*
Corresponding author. Mailing address: Laboratoire
ARAGO, BP44, F-66651 Banyuls-sur-Mer Cedex, France. Phone: (33) 4 68 88 73 53. Fax: (33) 4 68 88 73 95. E-mail:
lebaron{at}arago.obs-banyuls.fr.
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