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Appl Environ Microbiol, May 1998, p. 1766-1772, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Molecular Analysis of a Laccase Gene from the White Rot Fungus Pycnoporus cinnabarinus

Claudia Eggert,1,* Peter R. LaFayette,2 Ulrike Temp,1 Karl-Erik L. Eriksson,3 and Jeffrey F. D. Dean2

Institute of General and Microbial Genetics, Friedrich-Schiller University of Jena, 07743 Jena, Germany,1 and Department of Biochemistry and Molecular Biology, Center for Biological Resource Recovery,3 and Warnell School of Forest Resources,2 University of Georgia, Athens, Georgia 30602

Received 1 December 1997/Accepted 20 February 1998

It was recently shown that the white rot basidiomycete Pycnoporus cinnabarinus secretes an unusual set of phenoloxidases when it is grown under conditions that stimulate ligninolysis (C. Eggert, U. Temp, and K.-E. L. Eriksson, Appl. Environ. Microbiol. 62:1151-1158, 1996). In this report we describe the results of a cloning and structural analysis of the laccase-encoding gene (lcc3-1) expressed by P. cinnabarinus during growth under xylidine-induced conditions. The coding region of the genomic laccase sequence, which is preceded by the eukaryotic promoter elements TATA and CAATA, spans more than 2,390 bp. The corresponding laccase cDNA was identical to the genomic sequence except for 10 introns that were 50 to 60 bp long. A sequence analysis indicated that the P. cinnabarinus lcc3-1 product has a Phe residue at a position likely to influence the reduction-oxidation potential of the enzyme's type 1 copper center. The P. cinnabarinus lcc3-1 sequence was most similar to the sequence encoding a laccase from Coriolus hirsutus (level of similarity, 84%).

* Corresponding author. Mailing address: Institute of General Microbiology and Microbial Genetics, Friedrich-Schiller University of Jena, Neugasse 24, D-07743 Jena, Germany. Phone and fax: (49) 3641-949327. E-mail: Claudia.Eggert{at}uni-jena.de.

Appl Environ Microbiol, May 1998, p. 1766-1772, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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