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Appl Environ Microbiol, May 1998, p. 1890-1894, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Ecophysiological and Phylogenetic Studies of Nevskia ramosa in Pure Culture

Heike Stürmeyer,1,dagger Jörg Overmann,1 Hans-Dietrich Babenzien,2 and Heribert Cypionka1,*

Institut für Chemie und Biologie des Meeres, Oldenburg,1 and Institut für Gewässerökologie und Binnenfischerei, Neuglobsow,2 Germany

Received 4 September 1997/Accepted 17 February 1998

During the last 100 years, the neuston bacterium Nevskia ramosa has been described several times. This bacterium forms conspicuous rosette-like microcolonies at the air-water interface. In this study, pure cultures of Nevskia ramosa were obtained for the first time, from a bog lake (strain Soe1, DSMZ 11499T) and a freshwater ditch (strain OL1, DSMZ 11500). The isolates showed special adaptations to life in the epineuston. They formed hydrophobic surface films with a dull appearance. N. ramosa is sensitive to UV radiation but revealed a very effective photorepair mechanism. Exposure to light at a wavelength of 350 nm after UV treatment raised the number of surviving cells by several orders of magnitude. The isolates grew with a broad range of organic substrates. Surface films were formed only in the absence of combined nitrogen; however, nitrogenase activity was not detected. It appears that during growth at the air-water interface the cells benefit from trapping ammonia from the air. The G+C content of the DNA was 67.8 and 69.0 mol% for strains Soe1 and OL1, respectively. The slight difference was confirmed by enterobacterial repetitive intergenic consensus PCR. The 16S rRNA sequences revealed 99.2% similarity. Thus, both isolates belong to the same species. The phylogenetic analysis indicated that Nevskia is a member of the gamma-subclass Proteobacteria that has no known close relatives.

* Corresponding author. Mailing address: Institut für Chemie und Biologie des Meeres, Universität Oldenburg, Postfach 2503, D-26111 Oldenburg, Germany. Phone: 49 441 970 6360. Fax: 49 441 970 3583. E-mail: H.Cypionka{at}palmikro.icbm.uni-oldenburg.de.

dagger Present address: Max-Planck-Institut für marine Mikrobiologie, Bremen, Germany.

Appl Environ Microbiol, May 1998, p. 1890-1894, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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