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Appl Environ Microbiol, May 1998, p. 1919-1923, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

arfI and arfII, Two Genes Encoding alpha -L-Arabinofuranosidases in Cytophaga xylanolytica

Kwi S. Kim, Timothy G. Lilburn, Michael J. Renner, and John A. Breznak*

Department of Microbiology and Center for Microbial Ecology, Michigan State University, East Lansing, Michigan 48824-1101

Received 9 October 1997/Accepted 6 February 1998

arfI encoded the 57.7-kDa subunit of Cytophaga xylanolytica arabinofuranosidase I (ArfI). arfII encoded a 59.2-kDa subunit of ArfII. Products of both cloned genes liberated arabinose from arabinan and arabinoxylan. The deduced amino acid sequences of ArfI and ArfII revealed numerous regions that were identical to each other and to regions of homologous proteins from Bacteroides ovatus, Bacillus subtilis, and Clostridium stercorarium.

* Corresponding author. Mailing address: Department of Microbiology, Michigan State University, East Lansing, MI 48824-1101. Phone: (517) 355-6536. Fax: (517) 353-8957. E-mail: breznak{at}pilot.msu.edu.

Appl Environ Microbiol, May 1998, p. 1919-1923, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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