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Appl Environ Microbiol, May 1998, p. 1924-1928, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Phanerochaete chrysosporium Cellobiohydrolase and Cellobiose Dehydrogenase Transcripts in Wood

Marcelo A. Vallim,1 Bernard J. H. Janse,2 Jill Gaskell,3 Aline A. Pizzirani-Kleiner,1 and Daniel Cullen3,*

Departamento de Genetica, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, 13416-970, Piracicaba-São Paulo, Brazil1; Department of Microbiology, University of Stellenbosch, Stellenbosch 7602, South Africa2; and Institute for Microbial and Biochemical Technology, USDA Forest Products Laboratory, Madison, Wisconsin 537053

Received 26 September 1997/Accepted 30 January 1998

The transcripts of structurally related cellobiohydrolase genes in Phanerochaete chrysosporium-colonized wood chips were quantified. The transcript patterns obtained were dramatically different from the transcript patterns obtained previously in defined media. Cellobiose dehydrogenase transcripts were also detected, which is consistent with the hypothesis that such transcripts play an important role in cellulose degradation.


* Corresponding author. Mailing address: USDA Forest Products Laboratory, One Gifford Pinchot Drive, Madison, WI 53705. Phone: (608) 231-9468. Fax: (608) 231-9262. E-mail: dcullen{at}facstaff.wisc.edu.


Appl Environ Microbiol, May 1998, p. 1924-1928, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.



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