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Appl Environ Microbiol, May 1998, p. 1933-1936, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Chloromethane Metabolism by
Methylobacterium sp. Strain CM4
Todd
Vannelli,
Alex
Studer,
Michael
Kertesz, and
Thomas
Leisinger*
Mikrobiologisches Institut, ETH Zürich,
CH-8092 Zürich, Switzerland
Received 8 December 1997/Accepted 16 February 1998
Methylobacterium sp. strain CM4 metabolized
chloromethane quantitatively with a molar yield of 2.8 g of
whole-cell protein/mol of C. This value was similar to that observed
after growth with methanol (2.9 g of protein/mol of C) and about three
times larger than the yield with formate (0.94 g of protein/mol of C).
Chloromethane dehalogenation activity was inducible.
MiniTn5 transposon insertion mutants with altered growth
characteristics with chloromethane and other C1 compounds
were isolated and characterized. Nine of these were unable to grow with
chloromethane but were able to grow with methanol, methylamine, or
formate. Seventy-three transposon mutants that were defective in the
utilization of either methanol, methylamine, methanol plus methylamine,
or formate could still grow with chloromethane. Based on the protein
yield data and the properties of the transposon mutants, we propose a
pathway for chloromethane metabolism that depends on methyltransferase
and dehydrogenase activities.
*
Corresponding author. Mailing address:
Mikrobiologisches Institut, ETH-Zentrum, LFV, CH-8092 Zürich,
Switzerland. Phone: (41) 1 632 33 24. Fax: (41) 1 632 13 85. E-mail:
leisinger{at}micro.biol.ethz.ch.
Appl Environ Microbiol, May 1998, p. 1933-1936, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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