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Appl Environ Microbiol, May 1998, p. 1940-1946, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Structures of Homologous Composite Transposons Carrying cbaABC Genes from Europe and North America

Diana Di Gioia,1 Michelle Peel,2 Fabio Fava,1 and R. Campbell Wyndham2,*

Department of Applied Chemistry and Material Science, Faculty of Engineering, University of Bologna, 40136 Bologna, Italy,1 and Institute of Biology, Faculty of Science, Carleton University, Ottawa, Ontario K1S 5B6, Canada2

Received 29 October 1997/Accepted 15 January 1998

IS1071 is a class II transposable element carrying a tnpA gene related to the transposase genes of the Tn3 family. Copies of IS1071 that are conserved with more than 99% nucleotide sequence identity have been found as direct repeats flanking a remarkable variety of catabolic gene sequences worldwide. The sequences of chlorobenzoate catabolic transposons found on pBRC60 (Tn5271) in Niagara Falls, N.Y., and on pCPE3 in Bologna, Italy, show that these transposons were formed from highly homologous IS1071 and cbaABC components (levels of identity, >99.5 and >99.3%, respectively). Nevertheless, the junction sequences between the IS1071L and IS1071R elements and the internal DNA differ by 41 and 927 bp, respectively, suggesting that these transposons were assembled independently on the two plasmids. The formation of the right junction in both transposons truncated an open reading frame for a putative aryl-coenzyme A ligase with sequence similarity to benzoate- and p-hydroxybenzoate-coenzyme A ligases of Rhodopseudomonas palustris.

* Corresponding author. Mailing address: Institute of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario, Canada, K1S 5B6. Phone: (613) 520-2600, ext. 3651. Fax: (613) 520-3539. E-mail: cwyndham{at}ccs.carleton.ca.

Appl Environ Microbiol, May 1998, p. 1940-1946, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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