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Appl Environ Microbiol, May 1998, p. 1954-1957, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Differentiation of Cryptosporidium parvum Isolates by a Simplified Randomly Amplified Polymorphic DNA Technique

Ming Qi Deng and Dean O. Cliver*

Department of Population Health and Reproduction, School of Veterinary Medicine, University of California-Davis, Davis, California 95616

Received 6 October 1997/Accepted 9 March 1998

Genomic DNA was isolated from Cryptosporidium parvum oocysts by a specific immunomagnetic separation-in vitro excystation procedure and subjected to randomly amplified polymorphic DNA analysis using sequence-independent primers. An estuary C. parvum isolate was easily differentiated from several bovine isolates, while five bovine isolates of the same origin were indistinguishable from each other.

* Corresponding author. Mailing address: Department of Population Health and Reproduction, School of Veterinary Medicine, University of California at Davis, Davis, CA 95616. Phone: (530) 754-9120. Fax: (530) 752-5845. E-mail: docliver{at}ucdavis.edu.

Appl Environ Microbiol, May 1998, p. 1954-1957, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Limor, J. R., Lal, A. A., Xiao, L. (2002). Detection and Differentiation of Cryptosporidium Parasites That Are Pathogenic for Humans by Real-Time PCR. J. Clin. Microbiol. 40: 2335-2338 [Abstract] [Full Text]  
  • Straub, T. M., Daly, D. S., Wunshel, S., Rochelle, P. A., DeLeon, R., Chandler, D. P. (2002). Genotyping Cryptosporidium parvum with an hsp70 Single-Nucleotide Polymorphism Microarray. Appl. Environ. Microbiol. 68: 1817-1826 [Abstract] [Full Text]  


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