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Appl Environ Microbiol, May 1998, p. 1967-1971, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Endopolygalacturonase PG1 in Different Formae Speciales of Fusarium oxysporum

Antonio Di Pietro,* Fe I. García-Maceira, M. Dolores Huertas-González, M. Carmen Ruíz-Roldan, Zaira Caracuel, Andrea S. Barbieri,dagger and M. Isabel G. Roncero

Departamento de Genética, Facultad de Ciencias, Universidad de Córdoba, 14071 Córdoba, Spain

Received 21 October 1997/Accepted 19 February 1998

PG1, the major endopolygalacturonase of the vascular wilt pathogen Fusarium oxysporum, was secreted during growth on pectin by 10 of 12 isolates belonging to seven formae speciales, as determined with isoelectric focusing zymograms and sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels. A Southern analysis of genomic DNA and PCR performed with gene-specific primers revealed that the pg1 locus was highly conserved structurally in most isolates. Two PG1-deficient isolates were identified; one lacked the encoding gene, and the other carried a pg1 allele disrupted by a 3.2-kb insertion with sequence homology to hAT transposases. The virulence for muskmelon of different F. oxysporum f. sp. melonis isolates was not correlated with PG1 production in vitro. We concluded that PG1 is widely distributed in F. oxysporum and that it is not essential for pathogenicity.

* Corresponding author. Mailing address: Departamento de Genética, Facultad de Ciencias, Avda. de S. Alberto Magno s/n, 14071 Córdoba, Spain. Phone: 34-57218601. Fax: 34-57218606. E-mail: ge2dipia{at}uco.es.

dagger Present address: Dipartimento di Patologia Vegetale, Università degli Studi di Pisa, 56100 Pisa, Italy.

Appl Environ Microbiol, May 1998, p. 1967-1971, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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