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Appl Environ Microbiol, June 1998, p. 2141-2146, Vol. 64, No. 6
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cometabolism of 1,1-Dichloro-2,2-Bis(4-Chlorophenyl)Ethylene by Pseudomonas acidovorans M3GY Grown on Biphenyl

Anthony G. Haydagger and Dennis D. Focht*

Department of Soil and Environmental Sciences, University of California, Riverside, California 92521

Received 20 November 1997/Accepted 3 April 1998

1,1-Dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE), a toxic breakdown product of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT), has traditionally been viewed as a dead-end metabolite: there are no published reports detailing enzymatic ring fission of DDE by bacteria in either soil or pure culture. In this study, we investigated the ability of Pseudomonas acidovorans M3GY to transform DDE and its unchlorinated analog, 1,1-diphenylethylene (DPE). While strain M3GY could grow on DPE, cells grown on DPE as a sole carbon source could not degrade DDE. Cells grown on biphenyl, however, did degrade DDE. Mass balance analysis of [14C]DDE showed transformation of more than 40% of the recoverable radioactivity. Nine chlorinated metabolites produced from DDE were identified by gas chromatography-mass spectrometry-Fourier-transform infrared spectrometry (GC-MS-FTIR) from cultures grown on biphenyl. Recovery of these metabolites demonstrates that biphenyl-grown cells degrade DDE through a meta-fission pathway. This study provides a possible model for biodegradation of DDE in soil by biphenyl-utilizing bacteria.

* Corresponding author. Mailing address: Department of Plant Pathology, 2268 Geology Building, University of California, Riverside, CA 92521-0424. Phone: (909) 787-3446. Fax: (909) 787-3599. E-mail: Focht{at}CITRUS.UCR.EDU.

dagger Present address: Center for Environmental Biotechnology, University of Tennessee, Knoxville, Knoxville, TN 37996-1605.

Appl Environ Microbiol, June 1998, p. 2141-2146, Vol. 64, No. 6
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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