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Appl Environ Microbiol, June 1998, p. 2215-2219, Vol. 64, No. 6
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Isolation and Characterization of a Dibenzofuran-Degrading Yeast: Identification of Oxidation and Ring Cleavage Products

Elke Hammer,1,* Dirk Krowas,1 Annett Schäfer,1 Michael Specht,2 Wittko Francke,2 and Frieder Schauer1

Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universität, D-17489 Greifswald,1 and Institut für Organische Chemie, Universität Hamburg, D-20146 Hamburg,2 Federal Republic of Germany

Received 17 November 1997/Accepted 9 April 1998

We characterized the ability of a yeast to cleave the aromatic structure of the dioxin-like compound dibenzofuran. The yeast strain was isolated from a dioxin-contaminated soil sample and identified as Trichosporon mucoides. During incubation of glucose-pregrown cells with dibenzofuran, six major metabolites were detected by high-performance liquid chromatography. The formation of four different monohydroxylated dibenzofurans was proven by comparison of analytical data (gas chromatography-mass spectrometry) with that for authentic standards. Further oxidation produced 2,3-dihydroxydibenzofuran and its ring cleavage product 2-(1-carboxy methylidene)-2,3-dihydrobenzo[b]furanylidene glycolic acid, which were characterized by mass spectrometry and 1H nuclear magnetic resonance spectroscopy. These two metabolites are derived from 2-hydroxydibenzofuran and 3-hydroxydibenzofuran, as shown by incubation experiments using these monohydroxylated dibenzofurans as substrates.

* Corresponding author. Mailing address: Ernst-Moritz-Arndt-Universität Greifswald, Institut für Mikrobiologie und Molekularbiologie, F.-L.-Jahn-Str. 15, D-17487 Greifswald, Germany. Phone: 49-3834-864211. Fax: 49-3834-864202. E-mail: hammer{at}microbio1.biologie.uni-greifswald.de.

Appl Environ Microbiol, June 1998, p. 2215-2219, Vol. 64, No. 6
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sietmann, R., Hammer, E., Specht, M., Cerniglia, C. E., Schauer, F. (2001). Novel Ring Cleavage Products in the Biotransformation of Biphenyl by the Yeast Trichosporon mucoides. Appl. Environ. Microbiol. 67: 4158-4165 [Abstract] [Full Text]  
  • Gesell, M., Hammer, E., Specht, M., Francke, W., Schauer, F. (2001). Biotransformation of Biphenyl by Paecilomyces lilacinus and Characterization of Ring Cleavage Products. Appl. Environ. Microbiol. 67: 1551-1557 [Abstract] [Full Text]  
  • Becher, D., Specht, M., Hammer, E., Francke, W., Schauer, F. (2000). Cometabolic Degradation of Dibenzofuran by Biphenyl-Cultivated Ralstonia sp. Strain SBUG 290. Appl. Environ. Microbiol. 66: 4528-4531 [Abstract] [Full Text]  
  • Hundt, K., Martin, D., Hammer, E., Jonas, U., Kindermann, M. K., Schauer, F. (2000). Transformation of Triclosan by Trametes versicolor and Pycnoporus cinnabarinus. Appl. Environ. Microbiol. 66: 4157-4160 [Abstract] [Full Text]  


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