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Appl Environ Microbiol, June 1998, p. 2266-2268, Vol. 64, No. 6
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Estimation of Nitrifying Bacterial Activities by Measuring Oxygen Uptake in the Presence of the Metabolic Inhibitors Allylthiourea and Azide

Philippe Ginestet,1 Jean-Marc Audic,1,* Vincent Urbain,1 and Jean-Claude Block2

Centre International de Recherche sur l'Eau et l'Environment-Lyonnaise des Eaux, Le Pecq,1 and Laboratoire Santé-Environnement, UMR Université CNRS 7564, Faculté de Pharmacie, Nancy,2 France

Received 30 October 1997/Accepted 13 March 1998

The effects of two metabolic inhibitors on an enriched nitrifying biomass during incubation for short periods of time were investigated by determining respirometric measurements. Allylthiourea (86 µM) and azide (24 µM) were shown to be strong, selective inhibitors of ammonia and nitrite oxidation, respectively. Consequently, a differential respirometry method for estimating nitrifying and heterotrophic bacterial activities within a mixed biomass is proposed.

* Corresponding author. Mailing address: Division AB, CIRSEE-Lyonnaise des Eaux, 38 Rue du président Wilson, 78230 Le Pecq, France. Phone: 33-(0)1-34-80-23-45. Fax: 33-(0)1-30-53-62-11. E-mail: jmaudic{at}cirsee.suez-lyonnaise-eaux.fr.

Appl Environ Microbiol, June 1998, p. 2266-2268, Vol. 64, No. 6
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.