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Appl Environ Microbiol, June 1998, p. 2281-2283, Vol. 64, No. 6
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Method Detection Limits of PCR and Immunofluorescence Assay for Cryptosporidium parvum in Soil

Mark J. Walker,1,* Carlo Montemagno,2 Juliet C. Bryant,3 and William C. Ghiorse4

Department of Environmental and Resource Sciences, University of Nevada, Reno, Nevada 89557,1 and Department of Agricultural and Biological Engineering,2 Department of Animal Sciences,3 and Section of Microbiology, Division of Biological Sciences,4 Cornell University, Ithaca, New York 14853

Received 26 January 1998/Accepted 26 March 1998

We determined and compared the method detection limits (MDLalpha ) of a PCR and an immunofluorescence assay (IFA) for detection of Cryptosporidium parvum oocysts in soils. Based on the MDLalpha and the quantitative nature and stability of the IFA, PCR analysis is not a useful screening step for soil studies of oocyst transport.

* Corresponding author. Mailing address: Department of Environmental and Resource Sciences, University of Nevada, Reno, NV 89557-0013. Phone: (702) 784-6763. Fax: (702) 784-4789. E-mail: mwalker{at}equinox.unr.edu.

Appl Environ Microbiol, June 1998, p. 2281-2283, Vol. 64, No. 6
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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  • Walker, M., Redelman, D. (2004). Detection of Cryptosporidium parvum in Soil Extracts. Appl. Environ. Microbiol. 70: 1827-1829 [Abstract] [Full Text]  
  • Kuczynska, E., Shelton, D. R. (1999). Method for Detection and Enumeration of Cryptosporidium parvum Oocysts in Feces, Manures, and Soils. Appl. Environ. Microbiol. 65: 2820-2826 [Abstract] [Full Text]  
  • Jenkins, M. B., Walker, M. J., Bowman, D. D., Anthony, L. C., Ghiorse, W. C. (1999). Use of a Sentinel System for Field Measurements of Cryptosporidium parvum Oocyst Inactivation in Soil and Animal Waste. Appl. Environ. Microbiol. 65: 1998-2005 [Abstract] [Full Text]  


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