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Appl Environ Microbiol, June 1998, p. 2323-2326, Vol. 64, No. 6
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The atzABC Genes Encoding Atrazine Catabolism Are Located on a Self-Transmissible Plasmid in Pseudomonas sp. Strain ADPdagger

Mervyn L. de Souza,1,2,3 Lawrence P. Wackett,1,2,3,4 and Michael J. Sadowsky2,3,4,5,*

Department of Biochemistry,1 Department of Microbiology,4 Department of Soil, Water, and Climate,5 Biological Process Technology Institute,2 and Center for Biodegradation Research and Informatics,3 University of Minnesota, St. Paul, Minnesota 55108

Received 22 December 1997/Accepted 19 March 1998

Pseudomonas sp. strain ADP initiates atrazine catabolism via three enzymatic steps, encoded by atzA, -B, and -C, which yield cyanuric acid, a nitrogen source for many bacteria. In-well lysis, Southern hybridization, and plasmid transfer studies indicated that the atzA, -B, and -C genes are localized on a 96-kb self-transmissible plasmid, pADP-1, in Pseudomonas sp. strain ADP. High-performance liquid chromatography analyses showed that cyanuric acid degradation was not encoded by pADP-1. pADP-1 was transferred to Escherichia coli strains at a frequency of 4.7 × 10-2. This suggests a potential molecular mechanism for the dispersion of the atzABC genes to other soil bacteria.

* Corresponding author. Mailing address: Department of Soil, Water, and Climate, Biological Process Technology Institute and Center for Biodegradation Research and Informatics, University of Minnesota, 1991 Upper Buford Circle, Borlaug Hall, St. Paul, MN 55108. Phone: (612) 624-2706. Fax: (612) 625-2208. E-mail: sadowsky{at}soils.umn.edu.

dagger Article 981250043 in the University of Minnesota Agricultural Experiment Station series.

Appl Environ Microbiol, June 1998, p. 2323-2326, Vol. 64, No. 6
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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