This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Khan, A. A.
Right arrow Articles by Cerniglia, C. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Khan, A. A.
Right arrow Articles by Cerniglia, C. E.
Agricola
Right arrow Articles by Khan, A. A.
Right arrow Articles by Cerniglia, C. E.

 Previous Article  |  Next Article 

Appl Environ Microbiol, July 1998, p. 2473-2478, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Molecular Cloning, Nucleotide Sequence, and Expression in Escherichia coli of a Hemolytic Toxin (Aerolysin) Gene from Aeromonas trota

Ashraf A. Khan,* Eungbin Kim, and Carl E. Cerniglia

Division of Microbiology, Food and Drug Administration, National Center for Toxicological Research, Jefferson, Arkansas 72079

Received 22 January 1998/Accepted 16 April 1998

Aeromonas trota AK2, which was derived from ATCC 49659 and produces the extracellular pore-forming hemolytic toxin aerolysin, was mutagenized with the transposon mini-Tn5Km1 to generate a hemolysin-deficient mutant, designated strain AK253. Southern blotting data indicated that an 8.7-kb NotI fragment of the genomic DNA of strain AK253 contained the kanamycin resistance gene of mini-Tn5Km1. The 8.7-kb NotI DNA fragment was cloned into the vector pGEM5Zf(-) by selecting for kanamycin resistance, and the resultant clone, pAK71, showed aerolysin activity in Escherichia coli JM109. The nucleotide sequence of the aerA gene, located on the 1.8-kb ApaI-EcoRI fragment, was determined to consist of 1,479 bp and to have an ATG initiation codon and a TAA termination codon. An in vitro coupled transcription-translation analysis of the 1.8-kb region suggested that the aerA gene codes for a 54-kDa protein, in agreement with nucleotide sequence data. The deduced amino acid sequence of the aerA gene product of A. trota exhibited 99% homology with the amino acid sequence of the aerA product of Aeromonas sobria AB3 and 57% homology with the amino acid sequences of the products of the aerA genes of Aeromonas salmonicida 17-2 and A. sobria 33.

* Corresponding author. Mailing address: Division of Microbiology, U.S. Food and Drug Administration, National Center for Toxicological Research, Jefferson, AR 72079. Phone: (870) 543-7601. Fax: (870) 543-7307. E-mail: AKHAN{at}NCTR.FDA.GOV.

Appl Environ Microbiol, July 1998, p. 2473-2478, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kim, D., Kim, Y.-S., Kim, S.-K., Kim, S. W., Zylstra, G. J., Kim, Y. M., Kim, E. (2002). Monocyclic Aromatic Hydrocarbon Degradation by Rhodococcus sp. Strain DK17. Appl. Environ. Microbiol. 68: 3270-3278 [Abstract] [Full Text]  
  • Kingombe, C. I. B., Huys, G., Tonolla, M., Albert, M. J., Swings, J., Peduzzi, R., Jemmi, T. (1999). PCR Detection, Characterization, and Distribution of Virulence Genes in Aeromonas spp.. Appl. Environ. Microbiol. 65: 5293-5302 [Abstract] [Full Text]  
  • Santos, J. A., González, C. J., Otero, A., García-López, M.-L. (1999). Hemolytic Activity and Siderophore Production in Different Aeromonas Species Isolated from Fish. Appl. Environ. Microbiol. 65: 5612-5614 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»