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Appl Environ Microbiol, July 1998, p. 2686-2690, Vol. 64, No. 7
Institut für Molekulare
Infektionsbiologie,
Received 30 January 1998/Accepted 2 April 1998
Based on comparative sequence analysis, we have designed an
oligonucleotide probe complementary to a region of 16S rRNA of Legionella pneumophila which allows the differentiation of
L. pneumophila from other Legionella
species without cultivation. The specificity of the new probe, LEGPNE1,
was tested by in situ hybridization to a total of four serogroups
of six strains of L. pneumophila, five different
Legionella spp. and three nonlegionella species as reference strains. Furthermore, L. pneumophila
cells could be easily distinguished from Legionella
micdadei and Pseudomonas aeruginosa cells by using in
situ hybridization with probes LEGPNE1, LEG705, and EUB338 after
infection of the protozoan Acanthamoeba castellanii.
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Specific Detection of Legionella
pneumophila: Construction of a New 16S rRNA-Targeted
Oligonucleotide Probe
*
Corresponding author. Mailing address: Institut
für Molekulare Infektionsbiologie, Röntgenring 11, D-97070 Würzburg, Germany. Phone: 49-931-312150. Fax:
49-931-312578. E-mail:
bettina.brand{at}mail.uni-wuerzburg.de.
Appl Environ Microbiol, July 1998, p. 2686-2690, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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