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Appl Environ Microbiol, July 1998, p. 2686-2690, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Specific Detection of Legionella pneumophila: Construction of a New 16S rRNA-Targeted Oligonucleotide Probe

Dorothee Grimm,1 Hilde Merkert,1 Wolfgang Ludwig,2 Karl-Heinz Schleifer,2 Jörg Hacker,1 and Bettina C. Brand1,*

Institut für Molekulare Infektionsbiologie, D-97070 Würzburg,1 and Lehrstuhl für Mikrobiologie, Technische Universität München, D-80290 Munich,2 Germany

Received 30 January 1998/Accepted 2 April 1998

Based on comparative sequence analysis, we have designed an oligonucleotide probe complementary to a region of 16S rRNA of Legionella pneumophila which allows the differentiation of L. pneumophila from other Legionella species without cultivation. The specificity of the new probe, LEGPNE1, was tested by in situ hybridization to a total of four serogroups of six strains of L. pneumophila, five different Legionella spp. and three nonlegionella species as reference strains. Furthermore, L. pneumophila cells could be easily distinguished from Legionella micdadei and Pseudomonas aeruginosa cells by using in situ hybridization with probes LEGPNE1, LEG705, and EUB338 after infection of the protozoan Acanthamoeba castellanii.

* Corresponding author. Mailing address: Institut für Molekulare Infektionsbiologie, Röntgenring 11, D-97070 Würzburg, Germany. Phone: 49-931-312150. Fax: 49-931-312578. E-mail: bettina.brand{at}mail.uni-wuerzburg.de.

Appl Environ Microbiol, July 1998, p. 2686-2690, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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