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Appl Environ Microbiol, July 1998, p. 2691-2696, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Microbial Community Composition of Wadden Sea Sediments as Revealed by Fluorescence In Situ Hybridization

Enric Llobet-Brossa, Ramon Rosselló-Mora,* and Rudolf Amann

Max Planck Institut für Marine Mikrobiologie, 28359 Bremen, Germany

Received 25 February 1998/Accepted 14 April 1998

The microbial community composition of Wadden Sea sediments of the German North Sea coast was investigated by in situ hybridization with group-specific fluorescently labeled, rRNA-targeted oligonucleotides. A large fraction (up to 73%) of the DAPI (4',6-diamidino-2-phenylindole)-stained cells hybridized with the bacterial probes. Nearly 45% of the total cells could be further identified as belonging to known phyla. Members of the Cytophaga-Flavobacterium cluster were most abundant in all layers, followed by the sulfate-reducing bacteria.

* Corresponding author. Mailing address: Molecular Ecology Group, Max Planck Institut für marine Mikrobiologie, Celsiusstrasse 1, 28359 Bremen, Germany. Phone: 49-421-2028-940. Fax: 49-421-2028-580. E-mail: rrossell{at}mpi-bremen.de.

Appl Environ Microbiol, July 1998, p. 2691-2696, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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