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Appl Environ Microbiol, July 1998, p. 2697-2700, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Effectiveness of SYTOX Green Stain for Bacterial Viability Assessment

P. Lebaron,* P. Catala, and N. Parthuisot

Observatoire Océanologique, Centre National de la Recherche Scientifique (CNRS-UMR7621), Institut National des Sciences de l'Univers et Université Paris VI, F-66651 Banyuls-sur-Mer Cedex, France

Received 23 January 1998/Accepted 19 April 1998

The effectiveness of SYTOX Green nucleic acid stain for measuring bacterial viability was tested on starved populations of Escherichia coli and Salmonella typhimurium. This stain underestimates the fraction of dead cells within starved populations containing cells with damaged nucleic acids or membranes. Its application to natural samples should be considered with caution.

* Corresponding author. Mailing address: Laboratoire ARAGO, BP44, F-66651 Banyuls-sur-Mer Cedex, France. Phone: 33 4 68 88 73 53. Fax: 33 4 68 88 73 95. E-mail: lebaron{at}arago.obs-banyuls.fr.

Appl Environ Microbiol, July 1998, p. 2697-2700, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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