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Appl Environ Microbiol, July 1998, p. 2739-2742, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Microbial Community Changes in a Perturbed Agricultural Soil Investigated by Molecular and Physiological Approaches

Lise Øvreås,1 Sigmund Jensen,2,* Frida Lise Daae,1 and Vigdis Torsvik1

Department of Microbiology, University of Bergen, N-5020 Bergen,1 and Department of Biotechnological Sciences, Agricultural University of Norway, N-1432 Ås,2 Norway

Received 12 December 1997/Accepted 14 April 1998

Changes in soil microbial activity and diversity after incubation either with nitrogen or with a mixture of methane and air were examined. The perturbation by methane and air were characterized in detail and led to reduced diversity and enrichment of methanotrophs which were identified by denaturing gradient gel electrophoresis and 16S rRNA sequencing.

* Corresponding author. Mailing address: Department of Biotechnological Sciences, Agricultural University of Norway, Box 5040, N-1432 Ås, Norway. Phone: 47 64 947726. Fax: 47 64 947750. E-mail: sigmund.jensen{at}ibf.nlh.no.

Appl Environ Microbiol, July 1998, p. 2739-2742, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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