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Appl Environ Microbiol, July 1998, p. 2748-2754, Vol. 64, No. 7
Department of Biochemistry and Molecular
Biology, Oregon Graduate Institute of Science and Technology,
Portland, Oregon 97291-1000
Received 23 December 1997/Accepted 18 April 1998
Analysis of a 2.4-kb cDNA of the cellulose-binding extracellular
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Gene Cloning and Characterization of a Novel
Cellulose-Binding
-Glucosidase from Phanerochaete
chrysosporium
-glucosidase (CBGL) from Phanerochaete chrysosporium
suggested that CBGL is organized into two domains, an N-terminal
cellulose-binding domain and a C-terminal catalytic domain. Genomic
sequence analysis suggested that cbgl is encoded by 30 exons. Southern analysis of DNA from homokaryotic cultures indicated
that CBGL is encoded by two alleles, cbgl-1 and
cbgl-2, of a single gene.
*
Corresponding author. Mailing address: Department of
Biochemistry and Molecular Biology, Oregon Graduate Institute of
Science and Technology, P.O. Box 91000, Portland, OR 97291-1000. Phone: (503) 690-1134. Fax: (503) 690-1464. E-mail:
vreng{at}bmb.ogi.edu.
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