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Appl Environ Microbiol, July 1998, p. 2748-2754, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Gene Cloning and Characterization of a Novel Cellulose-Binding beta -Glucosidase from Phanerochaete chrysosporium

Bin Li and V. Renganathan*

Department of Biochemistry and Molecular Biology, Oregon Graduate Institute of Science and Technology, Portland, Oregon 97291-1000

Received 23 December 1997/Accepted 18 April 1998

Analysis of a 2.4-kb cDNA of the cellulose-binding extracellular beta -glucosidase (CBGL) from Phanerochaete chrysosporium suggested that CBGL is organized into two domains, an N-terminal cellulose-binding domain and a C-terminal catalytic domain. Genomic sequence analysis suggested that cbgl is encoded by 30 exons. Southern analysis of DNA from homokaryotic cultures indicated that CBGL is encoded by two alleles, cbgl-1 and cbgl-2, of a single gene.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Oregon Graduate Institute of Science and Technology, P.O. Box 91000, Portland, OR 97291-1000. Phone: (503) 690-1134. Fax: (503) 690-1464. E-mail: vreng{at}bmb.ogi.edu.

Appl Environ Microbiol, July 1998, p. 2748-2754, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Wymelenberg, A. V., Denman, S., Dietrich, D., Bassett, J., Yu, X., Atalla, R., Predki, P., Rudsander, U., Teeri, T. T., Cullen, D. (2002). Transcript Analysis of Genes Encoding a Family 61 Endoglucanase and a Putative Membrane-Anchored Family 9 Glycosyl Hydrolase from Phanerochaete chrysosporium. Appl. Environ. Microbiol. 68: 5765-5768 [Abstract] [Full Text]  
  • Stewart, P., Gaskell, J., Cullen, D. (2000). A Homokaryotic Derivative of a Phanerochaete chrysosporium Strain and Its Use in Genomic Analysis of Repetitive Elements. Appl. Environ. Microbiol. 66: 1629-1633 [Abstract] [Full Text]  


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