This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van Hylckama Vlieg, J. E. T.
Right arrow Articles by Janssen, D. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van Hylckama Vlieg, J. E. T.
Right arrow Articles by Janssen, D. B.
Agricola
Right arrow Articles by van Hylckama Vlieg, J. E. T.
Right arrow Articles by Janssen, D. B.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 1998, p. 2800-2805, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Glutathione S-Transferase with Activity towards cis-1,2-Dichloroepoxyethane Is Involved in Isoprene Utilization by Rhodococcus sp. Strain AD45

Johan E. T. van Hylckama Vlieg, Jaap Kingma, Arjan J. van den Wijngaard,dagger and Dick B. Janssen*

Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands

Received 27 February 1998/Accepted 12 May 1998

Rhodococcus sp. strain AD45 was isolated from an enrichment culture on isoprene (2-methyl-1,3-butadiene). Isoprene-grown cells of strain AD45 oxidized isoprene to 3,4-epoxy-3-methyl-1-butene, cis-1,2-dichloroethene to cis-1,2-dichloroepoxyethane, and trans-1,2-dichloroethene to trans-1,2-dichloroepoxyethane. Isoprene-grown cells also degraded cis-1,2-dichloroepoxyethane and trans-1,2-dichloroepoxyethane. All organic chlorine was liberated as chloride during degradation of cis-1,2-dichloroepoxyethane. A glutathione (GSH)-dependent activity towards 3,4-epoxy-3-methyl-1-butene, epoxypropane, cis-1,2-dichloroepoxyethane, and trans-1,2-dichloroepoxyethane was detected in cell extracts of cultures grown on isoprene and 3,4-epoxy-3-methyl-1-butene. The epoxide-degrading activity of strain AD45 was irreversibly lost upon incubation of cells with 1,2-epoxyhexane. A conjugate of GSH and 1,2-epoxyhexane was detected in cell extracts of cells exposed to 1,2-epoxyhexane, indicating that GSH is the physiological cofactor of the epoxide-transforming activity. The results indicate that a GSH S-transferase is involved in the metabolism of isoprene and that the enzyme can detoxify reactive epoxides produced by monooxygenation of chlorinated ethenes.

* Corresponding author. Mailing address: Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands. Phone: 31-50-3634209. Fax: 31-50-3634165. E-mail: d.b.janssen{at}chem.rug.nl.

dagger Present address: TNO Industrial Microbiology, 3700 AJ Zeist, The Netherlands.

Applied and Environmental Microbiology, August 1998, p. 2800-2805, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Jennings, L. K., Chartrand, M. M. G., Lacrampe-Couloume, G., Lollar, B. S., Spain, J. C., Gossett, J. M. (2009). Proteomic and Transcriptomic Analyses Reveal Genes Upregulated by cis-Dichloroethene in Polaromonas sp. Strain JS666. Appl. Environ. Microbiol. 75: 3733-3744 [Abstract] [Full Text]  
  • Krishnakumar, A. M., Sliwa, D., Endrizzi, J. A., Boyd, E. S., Ensign, S. A., Peters, J. W. (2008). Getting a Handle on the Role of Coenzyme M in Alkene Metabolism. Microbiol. Mol. Biol. Rev. 72: 445-456 [Abstract] [Full Text]  
  • Kanai, T., Takahashi, K., Inoue, H. (2006). Three Distinct-Type Glutathione S-Transferases from Escherichia coli Important for Defense against Oxidative Stress. J Biochem 140: 703-711 [Abstract] [Full Text]  
  • Danko, A. S., Saski, C. A., Tomkins, J. P., Freedman, D. L. (2006). Involvement of Coenzyme M during Aerobic Biodegradation of Vinyl Chloride and Ethene by Pseudomonas putida Strain AJ and Ochrobactrum sp. Strain TD.. Appl. Environ. Microbiol. 72: 3756-3758 [Abstract] [Full Text]  
  • Coleman, N. V., Spain, J. C. (2003). Epoxyalkane:Coenzyme M Transferase in the Ethene and Vinyl Chloride Biodegradation Pathways of Mycobacterium Strain JS60. J. Bacteriol. 185: 5536-5545 [Abstract] [Full Text]  
  • Coleman, N. V., Mattes, T. E., Gossett, J. M., Spain, J. C. (2002). Phylogenetic and Kinetic Diversity of Aerobic Vinyl Chloride-Assimilating Bacteria from Contaminated Sites. Appl. Environ. Microbiol. 68: 6162-6171 [Abstract] [Full Text]  
  • Coleman, N. V., Mattes, T. E., Gossett, J. M., Spain, J. C. (2002). Biodegradation of cis-Dichloroethene as the Sole Carbon Source by a {beta}-Proteobacterium. Appl. Environ. Microbiol. 68: 2726-2730 [Abstract] [Full Text]  
  • van Hylckama Vlieg, J. E. T., Leemhuis, H., Spelberg, J. H. L., Janssen, D. B. (2000). Characterization of the Gene Cluster Involved in Isoprene Metabolism in Rhodococcus sp. Strain AD45. J. Bacteriol. 182: 1956-1963 [Abstract] [Full Text]  
  • Bartels, F., Backhaus, S., Moore, E. R. B., Timmis, K. N., Hofer, B. (1999). Occurrence and expression of glutathione-S-transferase-encoding bphK genes in Burkholderia sp. strain LB400 and other biphenyl-utilizing bacteria. Microbiology 145: 2821-2834 [Abstract] [Full Text]  
  • van Hylckama Vlieg, J. E. T., Kingma, J., Kruizinga, W., Janssen, D. B. (1999). Purification of a Glutathione S-Transferase and a Glutathione Conjugate-Specific Dehydrogenase Involved in Isoprene Metabolism in Rhodococcus sp. Strain AD45. J. Bacteriol. 181: 2094-2101 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»