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Applied and Environmental Microbiology, August 1998, p. 2888-2893, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Ehrlichia risticii, the Agent of Potomac Horse Fever, in Freshwater Stream Snails (Pleuroceridae: Juga spp.) from Northern California

Jeffrey E. Barlough,1 Gerhard H. Reubel,1 John E. Madigan,1,* Larisa K. Vredevoe,2 Paul E. Miller,3 and Yasuko Rikihisa4

Department of Medicine and Epidemiology, School of Veterinary Medicine,1 and Department of Entomology,2 University of California, Davis, California 95616; 19305 Ordway Road, Weed, California 960943; and Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio 432104

Received 5 November 1997/Accepted 6 May 1998

Ehrlichia DNA was identified by nested PCR in operculate snails (Pleuroceridae: Juga spp.) collected from stream water in a northern California pasture in which Potomac horse fever (PHF) is enzootic. Sequencing of PCR-amplified DNA from a suite of genes (the 16S rRNA, groESL heat shock operon, 51-kDa major antigen genes) indicated that the source organism closely resembled Ehrlichia risticii, the causative agent of PHF. The minimum percentage of Juga spp. harboring the organism in the population studied was 3.5% (2 of 57 snails). No ehrlichia DNA was found in tissues of 123 lymnaeid, physid, and planorbid snails collected at the same site. These data suggest that pleurocerid stream snails may play a role in the life cycle of E. risticii in northern California.

* Corresponding author. Mailing address: School of Veterinary Medicine, University of California, Davis, CA 95616. Phone (530) 752-6513. Fax: (530) 752-0414. E-mail: jemadigan{at}ucdavis.edu.

Applied and Environmental Microbiology, August 1998, p. 2888-2893, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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