This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Barlough, J. E.
Right arrow Articles by Rikihisa, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barlough, J. E.
Right arrow Articles by Rikihisa, Y.
Agricola
Right arrow Articles by Barlough, J. E.
Right arrow Articles by Rikihisa, Y.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 1998, p. 2888-2893, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Ehrlichia risticii, the Agent of Potomac Horse Fever, in Freshwater Stream Snails (Pleuroceridae: Juga spp.) from Northern California

Jeffrey E. Barlough,1 Gerhard H. Reubel,1 John E. Madigan,1,* Larisa K. Vredevoe,2 Paul E. Miller,3 and Yasuko Rikihisa4

Department of Medicine and Epidemiology, School of Veterinary Medicine,1 and Department of Entomology,2 University of California, Davis, California 95616; 19305 Ordway Road, Weed, California 960943; and Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio 432104

Received 5 November 1997/Accepted 6 May 1998

Ehrlichia DNA was identified by nested PCR in operculate snails (Pleuroceridae: Juga spp.) collected from stream water in a northern California pasture in which Potomac horse fever (PHF) is enzootic. Sequencing of PCR-amplified DNA from a suite of genes (the 16S rRNA, groESL heat shock operon, 51-kDa major antigen genes) indicated that the source organism closely resembled Ehrlichia risticii, the causative agent of PHF. The minimum percentage of Juga spp. harboring the organism in the population studied was 3.5% (2 of 57 snails). No ehrlichia DNA was found in tissues of 123 lymnaeid, physid, and planorbid snails collected at the same site. These data suggest that pleurocerid stream snails may play a role in the life cycle of E. risticii in northern California.


* Corresponding author. Mailing address: School of Veterinary Medicine, University of California, Davis, CA 95616. Phone (530) 752-6513. Fax: (530) 752-0414. E-mail: jemadigan{at}ucdavis.edu.


Applied and Environmental Microbiology, August 1998, p. 2888-2893, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Rikihisa, Y., Zhang, C., Kanter, M., Cheng, Z., Ohashi, N., Fukuda, T. (2004). Analysis of p51, groESL, and the Major Antigen P51 in Various Species of Neorickettsia, an Obligatory Intracellular Bacterium That Infects Trematodes and Mammals. J. Clin. Microbiol. 42: 3823-3826 [Abstract] [Full Text]  
  • Mott, J., Muramatsu, Y., Seaton, E., Martin, C., Reed, S., Rikihisa, Y. (2002). Molecular Analysis of Neorickettsia risticii in Adult Aquatic Insects in Pennsylvania, in Horses Infected by Ingestion of Insects, and Isolated in Cell Culture. J. Clin. Microbiol. 40: 690-693 [Abstract] [Full Text]  
  • Kanter, M., Mott, J., Ohashi, N., Fried, B., Reed, S., Lin, Y. C., Rikihisa, Y. (2000). Analysis of 16S rRNA and 51-Kilodalton Antigen Gene and Transmission in Mice of Ehrlichia risticii in Virgulate Trematodes from Elimia livescens Snails in Ohio. J. Clin. Microbiol. 38: 3349-3358 [Abstract] [Full Text]  
  • Pusterla, N., Madigan, J. E., Chae, J.-S., DeRock, E., Johnson, E., Pusterla, J. B. (2000). Helminthic Transmission and Isolation of Ehrlichia risticii, the Causative Agent of Potomac Horse Fever, by Using Trematode Stages from Freshwater Stream Snails. J. Clin. Microbiol. 38: 1293-1297 [Abstract] [Full Text]