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Applied and Environmental Microbiology, August 1998, p. 2899-2905, Vol. 64, No. 8
U.S. Geological Survey, Menlo Park,
California 940251;
California Institute
of Technology, Pasadena, California 911252; and
Department of Chemical Engineering, University of Washington,
Seattle, Washington 981953
Received 5 March 1998/Accepted 2 June 1998
A facultatively methylotrophic bacterium, strain IMB-1, that has
been isolated from agricultural soil grows on methyl bromide (MeBr),
methyl iodide, methyl chloride, and methylated amines, as well as on
glucose, pyruvate, or acetate. Phylogenetic analysis of its 16S rRNA
gene sequence indicates that strain IMB-1 classes in the alpha subgroup
of the class Proteobacteria and is closely related to
members of the genus Rhizobium. The ability of strain IMB-1
to oxidize MeBr to CO2 is constitutive in cells regardless of the growth substrate. Addition of cell suspensions of strain IMB-1
to soils greatly accelerates the oxidation of MeBr, as does pretreatment of soils with low concentrations of methyl iodide. These
results suggest that soil treatment strategies can be devised whereby
bacteria can effectively consume MeBr during field fumigations, which
would diminish or eliminate the outward flux of MeBr to the atmosphere.
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Strain IMB-1, a Novel Bacterium for the Removal of
Methyl Bromide in Fumigated Agricultural Soils
*
Corresponding author. Mailing address: U.S. Geological
Survey, 345 Middlefield Rd., Menlo Park, CA 94025. Phone: (650)
329-4482. Fax: (650) 329-4463. E-mail: roremlan{at}usgs.gov.
Applied and Environmental Microbiology, August 1998, p. 2899-2905, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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