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Applied and Environmental Microbiology, August 1998, p. 2920-2924, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Novel beta -N-Acetylglucosaminidase from Streptomyces thermoviolaceus OPC-520: Gene Cloning, Expression, and Assignment to Family 3 of the Glycosyl Hydrolases

Hiroshi Tsujibo,* Naoya Hatano, Tadahisa Mikami, Ayako Hirasawa, Katsushiro Miyamoto, and Yoshihiko Inamori

Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan

Received 16 March 1998/Accepted 22 May 1998

A beta -N-acetylglucosaminidase gene (nagA) of Streptomyces thermoviolaceus OPC-520 was cloned in Streptomyces lividans 66. The nucleotide sequence of the gene, which encodes NagA, revealed an open reading frame of 1,896 bp, encoding a protein with an Mr of 66,329. The deduced primary structure of NagA was confirmed by comparison with the N-terminal amino acid sequence of the cloned beta -N-acetylglucosaminidase expressed by S. lividans. The enzyme shares no sequence similarity with the classical beta -N-acetylglucosaminidases belonging to family 20. However, NagA, which showed no detectable beta -glucosidase activity, revealed homology with microbial beta -glucosidases belonging to family 3; in particular, striking homology with the active-site regions of beta -glucosidases was observed. Thus, the above-mentioned results indicate that NagA from S. thermoviolaceus OPC-520 is classified as a family 3 glycosyl hydrolase. The enzyme activity was optimal at 60°C and pH 5.0, and the apparent Km and Vmax values for p-nitrophenyl-beta -N-acetylglucosamine were 425.7 µM and 24.8 µmol min-1 mg of protein-1, respectively.

* Corresponding author. Mailing address: Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan. Phone and fax: (81-726) 90-1057. E-mail: tsujibo{at}oysun01.oups.ac.jp.

Applied and Environmental Microbiology, August 1998, p. 2920-2924, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Shipkowski, S., Brenchley, J. E. (2005). Characterization of an Unusual Cold-Active {beta}-Glucosidase Belonging to Family 3 of the Glycoside Hydrolases from the Psychrophilic Isolate Paenibacillus sp. Strain C7. Appl. Environ. Microbiol. 71: 4225-4232 [Abstract] [Full Text]  
  • Faure, D. (2002). The Family-3 Glycoside Hydrolases: from Housekeeping Functions to Host-Microbe Interactions. Appl. Environ. Microbiol. 68: 1485-1490 [Full Text]  


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