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Applied and Environmental Microbiology, August 1998, p. 2977-2981, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Genetic Variation in the Free-Living Amoeba Naegleria fowleri

Michel Pélandakis,1,* Johan F. De Jonckheere,2 and Pierre Pernin1

Laboratoire de Biologie Cellulaire, Faculté de Pharmacie, 69373 Lyon cedex 08, France,1 and Protozoology Laboratory, Scientific Institute of Public Health, Louis Pasteur, B1050 Brussels, Belgium2

Received 23 February 1998/Accepted 15 May 1998

In this study, 30 strains of the pathogenic free-living amoeba Naegleria fowleri were investigated by using the randomly amplified polymorphic DNA (RAPD) method. The present study confirmed our previous finding that RAPD variation is not correlated with geographical origin. In particular, Mexican strains belong to the variant previously detected in Asia, Europe, and the United States. In France, surprisingly, strains from Cattenom gave RAPD patterns identical to those of the Japanese strains. In addition, all of these strains, together with an additional French strain from Chooz, exhibited similarities to South Pacific strains. The results also confirmed the presence of numerous variants in Europe, whereas only two variants were detected in the United States. The two variants found in the United States were different from the South Pacific variants. These findings do not support the previous hypothesis concerning the origin and modes of dispersal of N. fowleri.

* Corresponding author. Mailing address: Laboratoire de Biologie Cellulaire, Faculté de Pharmacie, 8 avenue Rockefeller, 69373 Lyon cedex 08, France. Phone: 04 78 77 71 54. Fax: 04 78 77 71 58. E-mail: pelandak{at}rockefeller1.univ-lyon1.fr.

Applied and Environmental Microbiology, August 1998, p. 2977-2981, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pelandakis, M., Pernin, P. (2002). Use of Multiplex PCR and PCR Restriction Enzyme Analysis for Detection and Exploration of the Variability in the Free-Living Amoeba Naegleria in the Environment. Appl. Environ. Microbiol. 68: 2061-2065 [Abstract] [Full Text]  


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