This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Marshall, S.
Right arrow Articles by Orrego, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marshall, S.
Right arrow Articles by Orrego, C.
Agricola
Right arrow Articles by Marshall, S.
Right arrow Articles by Orrego, C.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 1998, p. 3066-3069, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Minimally Invasive Detection of Piscirickettsia salmonis in Cultivated Salmonids via the PCR

Sergio Marshall,1 Sekou Heath,2 Vitalia Henríquez,1 and Cristián Orrego2,*

Instituto de Biología, Universidad Católica de Valparaíso, Valparaíso, Chile,1 and Conservation Genetics Laboratory, Department of Biology, San Francisco State University, San Francisco, California 941322

Received 22 September 1997/Accepted 8 May 1998

The attributes of the PCR allowed implementation of an assay for specific detection of Piscirickettsia salmonis from a few microliters of fish serum. This opens the way to less invasive modes of sampling for this microbial pathogen in salmonids.


* Corresponding author. Mailing address: Department of Biology, San Francisco State University, 1600 Holloway Avenue, San Francisco, CA 94132. Phone: (415) 338-3453. Fax: (415) 338-6245. E-mail: cob{at}sfsu.edu.


Applied and Environmental Microbiology, August 1998, p. 3066-3069, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Warsen, A. E., Krug, M. J., LaFrentz, S., Stanek, D. R., Loge, F. J., Call, D. R. (2004). Simultaneous Discrimination between 15 Fish Pathogens by Using 16S Ribosomal DNA PCR and DNA Microarrays. Appl. Environ. Microbiol. 70: 4216-4221 [Abstract] [Full Text]  
  • Reid, H. I., Griffen, A. A., Birkbeck, T. H. (2004). Isolates of Piscirickettsia salmonis from Scotland and Ireland Show Evidence of Clonal Diversity. Appl. Environ. Microbiol. 70: 4393-4397 [Abstract] [Full Text]  
  • Birkbeck, T. H., Griffen, A. A., Reid, H. I., Laidler, L. A., Wadsworth, S. (2004). Growth of Piscirickettsia salmonis to High Titers in Insect Tissue Culture Cells. Infect. Immun. 72: 3693-3694 [Abstract] [Full Text]  
  • Gonzalez, S. F., Krug, M. J., Nielsen, M. E., Santos, Y., Call, D. R. (2004). Simultaneous Detection of Marine Fish Pathogens by Using Multiplex PCR and a DNA Microarray. J. Clin. Microbiol. 42: 1414-1419 [Abstract] [Full Text]  
  • Henriquez, V., Rojas, M. V., Marshall, S. H. (2003). An Alternative Efficient Procedure for Purification of the Obligate Intracellular Fish Bacterial Pathogen Piscirickettsia salmonis. Appl. Environ. Microbiol. 69: 6268-6271 [Abstract] [Full Text]