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Applied and Environmental Microbiology, August 1998, p. 3075-3078, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Screening of a Fosmid Library of Marine Environmental Genomic DNA Fragments Reveals Four Clones Related to Members of the Order Planctomycetales

Kevin L. Vergin,1 Ena Urbach,1 Jeffery L. Stein,2 Edward F. DeLong,3 Brian D. Lanoil,1 and Stephen J. Giovannoni1,*

Department of Microbiology, Oregon State University, Corvallis, Oregon 973311; Diversa Corporation, San Diego, California 921212; and Monterey Bay Aquarium Research Institute, Moss Landing, California 950393

Received 24 November 1997/Accepted 22 May 1998

A fosmid library with inserts containing approximately 40 kb of marine bacterial DNA (J. L. Stein, T. L. Marsh, K. Y. Wu, H. Shizuya, and E. F. DeLong, J. Bacteriol. 178:591-599, 1996) yielded four clones with 16S rRNA genes from the order Planctomycetales. Three of the clones belong to the Pirellula group and one clone belongs to the Planctomyces group, based on phylogenetic and signature nucleotide analyses of full-length 16S rRNA genes. Sequence analysis of the ends of the genes revealed a consistent mismatch in a widely used bacterium-specific 16S rRNA PCR amplification priming site (27F), which has also been reported in some thermophiles and spirochetes.

* Corresponding author. Mailing address: Department of Microbiology, Nash Hall 220, Oregon State University, Corvallis, OR 97331-3804. Phone: (541) 737-1835. Fax: (541) 737-0496. E-mail: giovanns{at}bcc.orst.edu.

Applied and Environmental Microbiology, August 1998, p. 3075-3078, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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