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Applied and Environmental Microbiology, August 1998, p. 3099-3101, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparison of C18-Carboxypropylbetaine and Glass Bead DNA Extraction Methods for Detection of Mycobacterium bovis in Bovine Milk Samples and Analysis of Samples by PCR

Brandon J. Cornejo,1 Alfredo Sahagún-Ruiz,2 Francisco Suárez-Güemes,2 Charles G. Thornton,3 Thomas A. Ficht,4 and L. Garry Adams4,*

Department of Biology, Colorado State University, Fort Collins, Colorado 805231; Departamento de Microbiología Inmunología, Facultad de Medicina Veterinaria, Universidad Nacional Autónoma de México, México, Distrito Federal 04510, México2; Department of Molecular Biology & Genetics, Quest Diagnostics Incorporated, Baltimore, Maryland 212273; and Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843-44674

Received 23 January 1998/Accepted 3 June 1998

The purpose of this prospective study was to compare two different milk preparation methods to assay for the presence of Mycobacterium bovis by PCR. Detection by a C18-carboxypropylbetaine (CB-18)-based sample processing method was compared to extraction of DNA from milk with glass beads. Samples from 17 skin test-positive cattle were analyzed. Following CB-18 processing and glass bead extraction, the sensitivity of IS6110-based PCR was 94.1 and 58.8%, respectively (P < 0.025). Because CB-18 processing will permit the proficient use of PCR for diagnosis and surveillance of bovine tuberculosis, it will contribute to the more efficient detection and control of tuberculosis.

* Corresponding author. Mailing address: Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843-4467. Phone: (409) 862-4402. Fax: (409) 862-1088. E-mail: gadams{at}cvm.tamu.edu.

Applied and Environmental Microbiology, August 1998, p. 3099-3101, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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