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 Previous Article

Applied and Environmental Microbiology, August 1998, p. 3123-3126, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Effect of Humic Fractions and Clay on Biodegradation of Phenanthrene by a Pseudomonas fluorescens Strain Isolated from Soil

Jose-Julio Ortega-Calvo* and Cesareo Saiz-Jimenez

Instituto de Recursos Naturales y Agrobiologia, C.S.I.C., Apartado 1052, 41080 Seville, Spain

Received 4 February 1998/Accepted 2 June 1998

The mineralization of phenanthrene in pure cultures of a Pseudomonas fluorescens strain, isolated from soil, was measured in the presence of soil humic fractions and montmorillonite. Humic acid and clay, either separately or in combination, shortened the acclimation phase. A higher mineralization rate was measured in treatments with humic acid at 100 µg/ml. Humic acid at 10 µg/ml stimulated the transformation only in the presence of 10 g of clay per liter. We suggest that sorption of phenanthrene to these soil components may result in a higher concentration of substrate in the vicinity of the bacterial cells and therefore may increase its bioavailability.

* Corresponding author. Mailing address: Instituto de Recursos Naturales y Agrobiologia, C.S.I.C., Apartado 1052, 41080 Seville, Spain. Phone: 345 462 47 11. Fax: 345 462 40 02. E-mail: jjortega{at}irnase.csic.es.

Applied and Environmental Microbiology, August 1998, p. 3123-3126, Vol. 64, No. 8
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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