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Applied and Environmental Microbiology, September 1998, p. 3147-3152, Vol. 64, No. 9
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cloning and Expression of the Listeria monocytogenes Scott A ptsH and ptsI Genes, Coding for HPr and Enzyme I, Respectively, of the Phosphotransferase Systemdagger

Douglas P. Christensen,1,Dagger Andrew K. Benson,2 and Robert W. Hutkins2,*

School of Biological Sciences,1 and Department of Food Science and Technology,2 University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0919

Received 2 March 1998/Accepted 14 June 1998

The phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) utilizes high-energy phosphate present in PEP to drive the uptake of several different carbohydrates in bacteria. In order to examine the role of the PTS in the physiology of Listeria monocytogenes, we identified the ptsH and ptsI genes encoding the HPr and enzyme I proteins, respectively, of the PTS. Nucleotide sequence analysis indicated that the predicted proteins are nearly 70% similar to HPr and enzyme I proteins from other organisms. Purified L. monocytogenes HPr overexpressed in Escherichia coli was also capable of complementing an HPr defect in heterologous extracts of Staphylococcus aureus ptsH mutants. Additional studies of the transcriptional organization and control indicated that the ptsH and ptsI genes are organized into a transcription unit that is under the control of a consensus-like promoter and that expression of these genes is mediated by glucose availability and pH or by by-products of glucose metabolism.

* Corresponding author. Mailing address: Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, NE 68583-0919. Phone: (402) 472-2820. Fax: (402) 472-1693. E-mail: bhutkins{at}foodsci.unl.edu.

dagger Paper no. 12177 in the Journal Series of the Nebraska Agricultural Experiment Station, Lincoln.

Dagger Present address: Department of Food Science, North Carolina State University, Raleigh, NC 27695-7624.

Applied and Environmental Microbiology, September 1998, p. 3147-3152, Vol. 64, No. 9
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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