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Applied and Environmental Microbiology, September 1998, p. 3275-3281, Vol. 64, No. 9
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparative Studies of Class IIa Bacteriocins of Lactic Acid Bacteria

Vincent G. H. Eijsink,1,* Marianne Skeie,1 P. Hans Middelhoven,1,dagger May Bente Brurberg,2 and Ingolf F. Nes1

Laboratory of Microbial Gene Technology, Department of Biotechnological Sciences, Agricultural University of Norway,1 and The Norwegian Crop Research Institute,2 N-1432 Ås, Norway

Received 8 December 1997/Accepted 15 June 1998

Four class IIa bacteriocins (pediocin PA-1, enterocin A, sakacin P, and curvacin A) were purified to homogeneity and tested for activity toward a variety of indicator strains. Pediocin PA-1 and enterocin A inhibited more strains and had generally lower MICs than sakacin P and curvacin A. The antagonistic activity of pediocin-PA1 and enterocin A was much more sensitive to reduction of disulfide bonds than the antagonistic activity of sakacin P and curvacin A, suggesting that an extra disulfide bond that is present in the former two may contribute to their high levels of activity. The food pathogen Listeria monocytogenes was among the most sensitive indicator strains for all four bacteriocins. Enterocin A was most effective in inhibiting Listeria, having MICs in the range of 0.1 to 1 ng/ml. Sakacin P had the interesting property of being very active toward Listeria but not having concomitant high levels of activity toward lactic acid bacteria. Strains producing class IIa bacteriocins displayed various degrees of resistance toward noncognate class IIa bacteriocins; for the sakacin P producer, it was shown that this resistance is correlated with the expression of immunity genes. It is hypothesized that variation in the presence and/or expression of such immunity genes accounts in part for the remarkably large variation in bacteriocin sensitivity displayed by lactic acid bacteria.

* Corresponding author. Mailing address: Agricultural University of Norway, Department of Biotechnological Sciences, P.O. Box 5051, N-1432 Ås, Norway. Phone: 47-64949472. Fax: 47-64941465. E-mail: vincent.eijsink{at}ibf.nlh.no.

dagger Present address: CLB, 1006 AD Amsterdam, The Netherlands.

Applied and Environmental Microbiology, September 1998, p. 3275-3281, Vol. 64, No. 9
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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