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Applied and Environmental Microbiology, September 1998, p. 3383-3388, Vol. 64, No. 9
Center of Marine Biotechnology, University of
Maryland Biotechnology Institute, Baltimore, Maryland
212021;
Fachbereich Biologie/Chemie,
Universität Osnabrück, 49069 Osnabrück,
Germany2; and
Australian Institute
of Marine Science, Townsville, Queensland 4810, Australia3
Received 19 February 1998/Accepted 29 June 1998
The Streptomyces strains CHR3 and CHR28, isolated from
the Baltimore Inner Harbor, contained two and one, respectively, giant linear plasmids which carry terminally bound proteins. The plasmids pRJ3L (322 kb), from CHR3, and pRJ28 (330 kb), from CHR28, carry genes
homologous to the previously characterized chromosomal
Streptomyces lividans 66 operon encoding resistance against
mercuric compounds. Both plasmids are transmissible (without any
detectable rearrangement) to the chloramphenicol-resistant S. lividans TK24 strain lacking plasmids and carrying a chromosomal
deletion of the mer operon. S. lividans TK24
conjugants harboring pRJ3L or pRJ28 exhibited profiles of mercury
resistance to mercuric compounds similar to those of
Streptomyces strains CHR3 and CHR28.
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Mercury Resistance Is Encoded by Transferable Giant
Linear Plasmids in Two Chesapeake Bay Streptomyces
Strains
*
Corresponding author. Mailing address: Australian
Institute of Marine Science, PMB No. 3, Townsville, MC 4810 Queensland, Australia. Phone: 61-7-4753-4418. Fax: 61-7-4753-4285. E-mail: R.Hill{at}aims.gov.au
Contribution no. 302 from the Center of Marine Biotechnology.
Contribution no. 907 from the Australian Institute of Marine Science.
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