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Applied and Environmental Microbiology, September 1998, p. 3383-3388, Vol. 64, No. 9
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Mercury Resistance Is Encoded by Transferable Giant Linear Plasmids in Two Chesapeake Bay Streptomyces Strainsdagger

Jacques Ravel,1 Hildgund Schrempf,2 and Russell T. Hill1,3,*

Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 212021; Fachbereich Biologie/Chemie, Universität Osnabrück, 49069 Osnabrück, Germany2; and Australian Institute of Marine Science, Townsville, Queensland 4810, Australia3

Received 19 February 1998/Accepted 29 June 1998

The Streptomyces strains CHR3 and CHR28, isolated from the Baltimore Inner Harbor, contained two and one, respectively, giant linear plasmids which carry terminally bound proteins. The plasmids pRJ3L (322 kb), from CHR3, and pRJ28 (330 kb), from CHR28, carry genes homologous to the previously characterized chromosomal Streptomyces lividans 66 operon encoding resistance against mercuric compounds. Both plasmids are transmissible (without any detectable rearrangement) to the chloramphenicol-resistant S. lividans TK24 strain lacking plasmids and carrying a chromosomal deletion of the mer operon. S. lividans TK24 conjugants harboring pRJ3L or pRJ28 exhibited profiles of mercury resistance to mercuric compounds similar to those of Streptomyces strains CHR3 and CHR28.

* Corresponding author. Mailing address: Australian Institute of Marine Science, PMB No. 3, Townsville, MC 4810 Queensland, Australia. Phone: 61-7-4753-4418. Fax: 61-7-4753-4285. E-mail: R.Hill{at}aims.gov.au

dagger Contribution no. 302 from the Center of Marine Biotechnology. Contribution no. 907 from the Australian Institute of Marine Science.

Applied and Environmental Microbiology, September 1998, p. 3383-3388, Vol. 64, No. 9
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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