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Applied and Environmental Microbiology, September 1998, p. 3515-3519, Vol. 64, No. 9
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Composition and Susceptibility to Chlorhexidine of Multispecies Biofilms of Oral Bacteria

J. Pratten,1,* P. Barnett,2 and M. Wilson1

Department of Microbiology, Eastman Dental Institute, University of London, London WC1X 8LD,1 and SmithKline Beecham Consumer Healthcare, Weybridge, Surrey KT13 ODE,2 United Kingdom

Received 20 April 1998/Accepted 15 June 1998

Using a constant-depth film fermentor, we have grown a six-membered biofilm community with a bacterial composition similar to that found in supragingival dental plaque. Cryosectioning revealed the distribution of bacteria throughout the biofilm. Exposure to 0.2% chlorhexidine for up to 5 min had little effect on biofilm viability.

* Corresponding author. Mailing address: Department of Microbiology, Eastman Dental Institute, University of London, 256 Grays Inn Road, London WC1X 8LD, United Kingdom. Phone: (44) 171 915 1107. Fax: (44) 171 915 1127. E-mail: jpratten{at}eastman.ucl.ac.uk.

Applied and Environmental Microbiology, September 1998, p. 3515-3519, Vol. 64, No. 9
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lawrence, J. R., Zhu, B., Swerhone, G. D. W., Topp, E., Roy, J., Wassenaar, L. I., Rema, T., Korber, D. R. (2008). Community-Level Assessment of the Effects of the Broad-Spectrum Antimicrobial Chlorhexidine on the Outcome of River Microbial Biofilm Development. Appl. Environ. Microbiol. 74: 3541-3550 [Abstract] [Full Text]  
  • Rudney, J.D. (2000). Saliva and Dental Plaque. ADR 14: 29-39 [Abstract]  
  • Pratten, J., Bedi, R., Wilson, M. (2000). An In Vitro Study of the Effect of Fluoridated Milk on Oral Bacterial Biofilms. Appl. Environ. Microbiol. 66: 1720-1723 [Abstract] [Full Text]  
  • Pratten, J., Wilson, M. (1999). Antimicrobial Susceptibility and Composition of Microcosm Dental Plaques Supplemented with Sucrose. Antimicrob. Agents Chemother. 43: 1595-1599 [Abstract] [Full Text]  


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