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Applied and Environmental Microbiology, September 1998, p. 3536-3538, Vol. 64, No. 9
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Expression of Phanerochaete chrysosporium Genes Encoding Lignin Peroxidases, Manganese Peroxidases, and Glyoxal Oxidase in Wood

Bernard J. H. Janse,1 Jill Gaskell,2 Masood Akhtar,2,3 and Daniel Cullen2,*

Department of Microbiology, University of Stellenbosch, Stellenbosch, 7600 South Africa,1 and Institute of Microbial and Biochemical Technology, USDA Forest Products Laboratory,2 and University of Wisconsin Biotechnology Center,3 Madison, Wisconsin 53706

Received 20 April 1998/Accepted 23 June 1998

Expression of Phanerochaete chrysosporium genes encoding ligninolytic enzymes was assessed in wood. Poly(A) RNA was extracted from colonized wood chips by magnetic capture, and specific transcripts were quantified by competitive reverse transcriptase PCR. mRNA levels varied substantially among lignin peroxidase genes, and transcript patterns were dramatically different from those in previous studies with defined media.

* Corresponding author. Mailing address: USDA Forest Products Laboratory, One Gifford Pinchot Drive, Madison, WI 53705. Phone: (608) 231-9468. Fax: (608) 231-9488. E-mail: dcullen{at}facstaff.wisc.edu.

Applied and Environmental Microbiology, September 1998, p. 3536-3538, Vol. 64, No. 9
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.