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Applied and Environmental Microbiology, September 1998, p. 3536-3538, Vol. 64, No. 9
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Expression of Phanerochaete
chrysosporium Genes Encoding Lignin Peroxidases, Manganese
Peroxidases, and Glyoxal Oxidase in Wood
Bernard J. H.
Janse,1
Jill
Gaskell,2
Masood
Akhtar,2,3 and
Daniel
Cullen2,*
Department of Microbiology, University of
Stellenbosch, Stellenbosch, 7600 South Africa,1
and
Institute of Microbial and Biochemical Technology, USDA
Forest Products Laboratory,2 and
University of Wisconsin Biotechnology
Center,3 Madison, Wisconsin 53706
Received 20 April 1998/Accepted 23 June 1998
Expression of Phanerochaete chrysosporium genes
encoding ligninolytic enzymes was assessed in wood. Poly(A) RNA was
extracted from colonized wood chips by magnetic capture, and specific
transcripts were quantified by competitive reverse transcriptase PCR.
mRNA levels varied substantially among lignin peroxidase genes, and transcript patterns were dramatically different from those in previous
studies with defined media.
*
Corresponding author. Mailing address: USDA Forest
Products Laboratory, One Gifford Pinchot Drive, Madison, WI 53705. Phone: (608) 231-9468. Fax: (608) 231-9488. E-mail:
dcullen{at}facstaff.wisc.edu.
Applied and Environmental Microbiology, September 1998, p. 3536-3538, Vol. 64, No. 9
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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