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Applied and Environmental Microbiology, January 1999, p. 278-282, Vol. 65, No. 1
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

New Mobilizable Vectors Suitable for Gene Replacement in Gram-Negative Bacteria and Their Use in Mapping of the 3' End of the Xanthomonas campestris pv. campestris gum Operon

Federico Katzen,1 Anke Becker,2 M. Verónica Ielmini,1 Cristian G. Oddo,1 and Luis Ielpi1,*

Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, UBA, and CONICET, 1405 Buenos Aires, Argentina,1 and Lehrstuhl für Genetik, Universität Bielefeld, D-33501 Bielefeld, Germany2

Received 20 July 1998/Accepted 29 October 1998

We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mapping the 3' end of the Xanthomonas campestris gum operon, involved in biosynthesis of xanthan.

* Corresponding author. Mailing address: Instituto de Investigaciones Bioquímicas Fundación Campomar, Patricias Argentinas 435, 1405 Buenos Aires, Argentina. Phone: 54(1) 863-4011/19. Fax: 54(1) 865-2246. E-mail: LIELPI{at}iib.uba.ar.

Applied and Environmental Microbiology, January 1999, p. 278-282, Vol. 65, No. 1
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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