Applied and Environmental Microbiology, January 1999, p. 278-282, Vol. 65, No. 1
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, UBA, and CONICET, 1405 Buenos Aires, Argentina,1 and Lehrstuhl für Genetik, Universität Bielefeld, D-33501 Bielefeld, Germany2
Received 20 July 1998/Accepted 29 October 1998
We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mapping the 3' end of the Xanthomonas campestris gum operon, involved in biosynthesis of xanthan.
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