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Applied and Environmental Microbiology, January 1999, p. 278-282, Vol. 65, No. 1
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
New Mobilizable Vectors Suitable for Gene Replacement in
Gram-Negative Bacteria and Their Use in Mapping of the 3' End of the
Xanthomonas campestris pv. campestris
gum Operon
Federico
Katzen,1
Anke
Becker,2
M. Verónica
Ielmini,1
Cristian G.
Oddo,1 and
Luis
Ielpi1,*
Instituto de Investigaciones
Bioquímicas Fundación Campomar, Facultad de Ciencias
Exactas y Naturales, UBA, and CONICET, 1405 Buenos Aires,
Argentina,1 and
Lehrstuhl für
Genetik, Universität Bielefeld, D-33501 Bielefeld,
Germany2
Received 20 July 1998/Accepted 29 October 1998
We describe useful vectors to select double-crossover events
directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene,
providing colorimetric screens to identify bacteria harboring those
sequences. The applicability of these vectors was shown by mapping the
3' end of the Xanthomonas campestris gum operon, involved
in biosynthesis of xanthan.
*
Corresponding author. Mailing address: Instituto
de Investigaciones Bioquímicas Fundación
Campomar, Patricias Argentinas 435, 1405 Buenos Aires, Argentina.
Phone: 54(1) 863-4011/19. Fax: 54(1) 865-2246. E-mail:
LIELPI{at}iib.uba.ar.
Applied and Environmental Microbiology, January 1999, p. 278-282, Vol. 65, No. 1
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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