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Applied and Environmental Microbiology, January 1999, p. 283-286, Vol. 65, No. 1
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Phylogenetic Analysis of an Anaerobic, Trichlorobenzene-Transforming Microbial Consortium

Friedrich von Wintzingerode,1 Burkhard Selent,2 Werner Hegemann,2 and Ulf B. Göbel1,*

Institut für Mikrobiologie und Hygiene, Universitätsklinikum Charité, 10117 Berlin,1 and Institut für Technischen Umweltschutz, Technische Universität Berlin, 10623 Berlin,2 Germany

Received 3 August 1998/Accepted 22 October 1998

A culture-independent phylogenetic survey for an anaerobic trichlorobenzene-transforming microbial community was carried out. Small-subunit rRNA genes were PCR amplified from community DNA by using primers specific for Bacteria or Euryarchaeota and were subsequently cloned. Application of a new hybridization-based screening approach revealed 51 bacterial clone families, one of which was closely related to dechlorinating Dehalobacter species. Several clone sequences clustered to rDNA sequences obtained from a molecular study of an anaerobic aquifer contaminated with hydrocarbons and chlorinated solvents (Dojka et al., Appl. Env. Microbiol. 64:3869-3877, 1998).

* Corresponding author. Mailing address: Institut für Mikrobiologie und Hygiene, Universitätsklinikum Charité, Dorotheenstr. 96, 10117 Berlin, Germany. Phone: 49-30-20934715. Fax: 49-30-20934703. E-mail: ulf.goebel{at}charite.de.

Applied and Environmental Microbiology, January 1999, p. 283-286, Vol. 65, No. 1
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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