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Applied and Environmental Microbiology, January 1999, p. 294-296, Vol. 65, No. 1
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Organic Solvent Tolerance of Escherichia coli Is Independent of OmpF Levels in the Membrane

Hiroyuki Asako, Kei Kobayashi, and Rikizo Aono*

Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta 4259, Midori-ku, Yokohama 226-8501, Japan

Received 17 August 1998/Accepted 9 October 1998

The organic solvent tolerance of Escherichia coli was measured under conditions in which OmpF levels were controlled by various means as follows: alteration of NaCl concentration in the medium, transformation with a stress-responsive gene (marA, robA, or soxS), or disruption of the ompF gene. It was shown that solvent tolerance of E. coli did not depend upon OmpF levels in the membrane.

* Corresponding author. Mailing address: Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta 4259, Midori-ku, Yokohama 226-8501, Japan. Phone: (81) 45-924-5766. Fax: (81) 45-924-5819. E-mail: raono{at}bio.titech.ac.jp.

Applied and Environmental Microbiology, January 1999, p. 294-296, Vol. 65, No. 1
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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