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Applied and Environmental Microbiology, January 1999, p. 346-350, Vol. 65, No. 1
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Development of a PCR Assay for Detection of Yersinia ruckeri in Tissues of Inoculated and Naturally Infected Trout

A. Gibello, M. M. Blanco, M. A. Moreno, M. T. Cutuli, A. Domenech, L. Domínguez, and J. F. Fernández-Garayzábal*

Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain

Received 13 July 1998/Accepted 22 October 1998

A PCR-based method was developed for the specific detection of Yersinia ruckeri in tissues of inoculated trout and naturally infected trout. No amplification products were obtained with other yersiniae, bacterial fish pathogens, or phylogenetically related bacteria (n = 34). The sensitivity of PCR detection was 60 to 65 bacterial cells per PCR tube, which was decreased to 10 to 20 cells by hybridization with a nonradioactive probe. The PCR assay proved to be as reliable as and faster than the conventional culture method for the detection of Y. ruckeri in infected trout tissues.

* Corresponding author. Mailing address: Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain. Phone: 34 91 3943716. Fax: 34 91 3943908. E-mail: garayzab{at}eucmax.sim.ucm.es.

Applied and Environmental Microbiology, January 1999, p. 346-350, Vol. 65, No. 1
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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