This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Alander, M.
Right arrow Articles by von Wright, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Alander, M.
Right arrow Articles by von Wright, A.
Agricola
Right arrow Articles by Alander, M.
Right arrow Articles by von Wright, A.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 1999, p. 351-354, Vol. 65, No. 1
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Persistence of Colonization of Human Colonic Mucosa by a Probiotic Strain, Lactobacillus rhamnosus GG, after Oral Consumption

Minna Alander,1 Reetta Satokari,1 Riitta Korpela,2 Maija Saxelin,2 Terttu Vilpponen-Salmela,3 Tiina Mattila-Sandholm,1 and Atte von Wright1,*

VTT Biotechnology and Food Research, FIN-02044 VTT,1 Valio Ltd. Research and Development Centre, FIN-00101 Helsinki,2 and Harjula Hospital, FIN-70101 Kuopio,3 Finland

Received 8 June 1998/Accepted 30 September 1998

Lactobacillus rhamnosus GG is one of the most thoroughly studied probiotic strains. Its advantages in the treatment of gastrointestinal disorders are well documented. The aim of the present study was to demonstrate with colonic biopsies the attachment of strain GG to human intestinal mucosae and the persistence of the attachment after discontinuation of GG administration. A whey drink fermented with strain GG was fed to human volunteers for 12 days. Fecal samples were collected before, during, and after consumption. L. rhamnosus GG-like colonies were detected in both fecal and colonic biopsy samples. Strain GG was identified by its characteristic colony morphology, a lactose fermentation test, and PCR. This study showed that strain GG was able to attach in vivo to colonic mucosae and, although the attachment was temporary, to remain for more than a week after discontinuation of GG administration. The results demonstrate that the study of fecal samples alone is not sufficient in evaluating colonization by a probiotic strain.

* Corresponding author. Present address: University of Kuopio, Department of Biochemistry and Biotechnology, P.O. Box 1627, FIN-70211 Kuopio, Finland. Phone: 358 17 162087. Fax: 358 17 2811510. E-mail: Atte.vonWright{at}uku.fi.

Applied and Environmental Microbiology, January 1999, p. 351-354, Vol. 65, No. 1
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Tsai, Y.-K., Chen, H.-W., Lo, T.-C., Lin, T.-H. (2009). Specific point mutations in Lactobacillus casei ATCC 27139 cause a phenotype switch from Lac- to Lac+. Microbiology 155: 751-760 [Abstract] [Full Text]  
  • Lebeer, S., Claes, I. J. J., Verhoeven, T. L. A., Shen, C., Lambrichts, I., Ceuppens, J. L., Vanderleyden, J., De Keersmaecker, S. C. J. (2008). Impact of luxS and Suppressor Mutations on the Gastrointestinal Transit of Lactobacillus rhamnosus GG. Appl. Environ. Microbiol. 74: 4711-4718 [Abstract] [Full Text]  
  • Farnworth, E. R. (2008). The Evidence to Support Health Claims for Probiotics. J. Nutr. 138: 1250S-1254S [Abstract] [Full Text]  
  • Guglielmetti, S., De Noni, I., Caracciolo, F., Molinari, F., Parini, C., Mora, D. (2008). Bacterial Cinnamoyl Esterase Activity Screening for the Production of a Novel Functional Food Product. Appl. Environ. Microbiol. 74: 1284-1288 [Abstract] [Full Text]  
  • Lebeer, S., Verhoeven, T. L. A., Perea Velez, M., Vanderleyden, J., De Keersmaecker, S. C. J. (2007). Impact of Environmental and Genetic Factors on Biofilm Formation by the Probiotic Strain Lactobacillus rhamnosus GG. Appl. Environ. Microbiol. 73: 6768-6775 [Abstract] [Full Text]  
  • Xu, Y., Li, Y. (2007). Induction of Immune Responses in Mice after Intragastric Administration of Lactobacillus casei Producing Porcine Parvovirus VP2 Protein. Appl. Environ. Microbiol. 73: 7041-7047 [Abstract] [Full Text]  
  • Galdeano, C. M., de Moreno de LeBlanc, A., Vinderola, G., Bonet, M. E. B., Perdigon, G. (2007). Proposed Model: Mechanisms of Immunomodulation Induced by Probiotic Bacteria. CVI 14: 485-492 [Full Text]  
  • Oozeer, R., Leplingard, A., Mater, D. D. G., Mogenet, A., Michelin, R., Seksek, I., Marteau, P., Dore, J., Bresson, J.-L., Corthier, G. (2006). Survival of Lactobacillus casei in the Human Digestive Tract after Consumption of Fermented Milk. Appl. Environ. Microbiol. 72: 5615-5617 [Abstract] [Full Text]  
  • Boyle, R. J, Robins-Browne, R. M, Tang, M. L. (2006). Probiotic use in clinical practice: what are the risks?. Am. J. Clin. Nutr. 83: 1256-1264 [Abstract] [Full Text]  
  • Teo, A. Y.-L., Tan, H.-M. (2005). Inhibition of Clostridium perfringens by a Novel Strain of Bacillus subtilis Isolated from the Gastrointestinal Tracts of Healthy Chickens. Appl. Environ. Microbiol. 71: 4185-4190 [Abstract] [Full Text]  
  • Lavermicocca, P., Valerio, F., Lonigro, S. L., De Angelis, M., Morelli, L., Callegari, M. L., Rizzello, C. G., Visconti, A. (2005). Study of Adhesion and Survival of Lactobacilli and Bifidobacteria on Table Olives with the Aim of Formulating a New Probiotic Food. Appl. Environ. Microbiol. 71: 4233-4240 [Abstract] [Full Text]  
  • Salminen, S. J., Gueimonde, M., Isolauri, E. (2005). Probiotics That Modify Disease Risk. J. Nutr. 135: 1294-1298 [Abstract] [Full Text]  
  • Suokko, A., Savijoki, K., Malinen, E., Palva, A., Varmanen, P. (2005). Characterization of a Mobile clpL Gene from Lactobacillus rhamnosus. Appl. Environ. Microbiol. 71: 2061-2069 [Abstract] [Full Text]  
  • Dickson, E M, Riggio, M P, Macpherson, L (2005). A novel species-specific PCR assay for identifying Lactobacillus fermentum. J Med Microbiol 54: 299-303 [Abstract] [Full Text]  
  • Baharav, E., Mor, F., Halpern, M., Weinberger, A. (2004). Lactobacillus GG Bacteria Ameliorate Arthritis in Lewis Rats. J. Nutr. 134: 1964-1969 [Abstract] [Full Text]  
  • Tannock, G. W. (2004). A Special Fondness for Lactobacilli. Appl. Environ. Microbiol. 70: 3189-3194 [Full Text]  
  • Drakes, M., Blanchard, T., Czinn, S. (2004). Bacterial Probiotic Modulation of Dendritic Cells. Infect. Immun. 72: 3299-3309 [Abstract] [Full Text]  
  • Lee, Y. K., Ho, P. S., Low, C. S., Arvilommi, H., Salminen, S. (2004). Permanent Colonization by Lactobacillus casei Is Hindered by the Low Rate of Cell Division in Mouse Gut. Appl. Environ. Microbiol. 70: 670-674 [Abstract] [Full Text]  
  • De Champs, C., Maroncle, N., Balestrino, D., Rich, C., Forestier, C. (2003). Persistence of Colonization of Intestinal Mucosa by a Probiotic Strain, Lactobacillus casei subsp. rhamnosus Lcr35, after Oral Consumption. J. Clin. Microbiol. 41: 1270-1273 [Abstract] [Full Text]  
  • Zoetendal, E. G., von Wright, A., Vilpponen-Salmela, T., Ben-Amor, K., Akkermans, A. D. L., de Vos, W. M. (2002). Mucosa-Associated Bacteria in the Human Gastrointestinal Tract Are Uniformly Distributed along the Colon and Differ from the Community Recovered from Feces. Appl. Environ. Microbiol. 68: 3401-3407 [Abstract] [Full Text]  
  • Nakayama, J., Kariyama, R., Kumon, H. (2002). Description of a 23.9-Kilobase Chromosomal Deletion Containing a Region Encoding fsr Genes Which Mainly Determines the Gelatinase-Negative Phenotype of Clinical Isolates of Enterococcus faecalis in Urine. Appl. Environ. Microbiol. 68: 3152-3155 [Abstract] [Full Text]  
  • Moser, S. A., Savage, D. C. (2001). Bile Salt Hydrolase Activity and Resistance to Toxicity of Conjugated Bile Salts Are Unrelated Properties in Lactobacilli. Appl. Environ. Microbiol. 67: 3476-3480 [Abstract] [Full Text]  
  • Vanderhoof, J. A (2001). Probiotics: future directions. Am. J. Clin. Nutr. 73 : 1152S-1155S [Abstract] [Full Text]  
  • Satokari, R. M., Vaughan, E. E., Akkermans, A. D. L., Saarela, M., de Vos, W. M. (2001). Bifidobacterial Diversity in Human Feces Detected by Genus-Specific PCR and Denaturing Gradient Gel Electrophoresis. Appl. Environ. Microbiol. 67: 504-513 [Abstract] [Full Text]  
  • Jacobsen, C. N., Rosenfeldt Nielsen, V., Hayford, A. E., Møller, P. L., Michaelsen, K. F., Pærregaard, A., Sandström, B., Tvede, M., Jakobsen, M. (1999). Screening of Probiotic Activities of Forty-Seven Strains of Lactobacillus spp. by In Vitro Techniques and Evaluation of the Colonization Ability of Five Selected Strains in Humans. Appl. Environ. Microbiol. 65: 4949-4956 [Abstract] [Full Text]  
  • Tynkkynen, S., Satokari, R., Saarela, M., Mattila-Sandholm, T., Saxelin, M. (1999). Comparison of Ribotyping, Randomly Amplified Polymorphic DNA Analysis, and Pulsed-Field Gel Electrophoresis in Typing of Lactobacillus rhamnosus and L. casei Strains. Appl. Environ. Microbiol. 65: 3908-3914 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»