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Applied and Environmental Microbiology, October 1999, p. 4313-4319, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of Bacillus Spores by Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry

Yetrib Hathout,1,* Plamen A. Demirev,1 Yen-Peng Ho,1 Jonathan L. Bundy,1 Victor Ryzhov,1 Lisa Sapp,1 James Stutler,2 Joany Jackman,3 and Catherine Fenselau1

Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 207421; GeoCenters, Inc., Fort Detrick, Maryland 217022; and Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, D.C.3

Received 27 April 1999/Accepted 16 July 1999

Unique patterns of biomarkers were reproducibly characterized by matrix-assisted laser desorption ionization (MALDI)-mass spectrometry and were used to distinguish Bacillus species members from one another. Discrimination at the strain level was demonstrated for Bacillus cereus spores. Lipophilic biomarkers were invariant in Bacillus globigii spores produced in three different media and in B. globigii spores stored for more than 30 years. The sensitivity was less than 5,000 cells deposited for analysis. Protein biomarkers were also characterized by MALDI analysis by using spores treated briefly with corona plasma discharge. Protein biomarkers were readily desorbed following this treatment. The effect of corona plasma discharge on the spores was examined.

* Corresponding author. Mailing address: Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742. Phone: (301) 405-8614. Fax: (301) 405-8415. E-mail: hathout{at}wam.umd.edu.

Applied and Environmental Microbiology, October 1999, p. 4313-4319, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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