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Applied and Environmental Microbiology, October 1999, p. 4329-4333, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Outer Membrane of Gram-Negative Bacteria Inhibits Antibacterial Activity of Brochocin-C

Yan Gao, Marco J. van Belkum, and Michael E. Stiles*

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5

Received 15 March 1999/Accepted 21 July 1999

Brochocin-C is a two-peptide bacteriocin produced by Brochothrix campestris ATCC 43754 that has a broad activity spectrum comparable to that of nisin. Brochocin-C has an inhibitory effect on EDTA-treated gram-negative bacteria, Salmonella enterica serovar Typhimurium lipopolysaccharide mutants, and spheroplasts of Typhimurium strains LT2 and SL3600. Brochocin-C treatment of cells and spheroplasts of strains of LT2 and SL3600 resulted in hydrolysis of ATP. The outer membrane of gram-negative bacteria protects the cytoplasmic membrane from the action of brochocin-C. It appears that brochocin-C is similar to nisin and possibly does not require a membrane receptor for its function; however, the difference in effect of the two bacteriocins on intracellular ATP indicates that they cause different pore sizes in the cytoplasmic membrane.

* Corresponding author. Mailing address: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada. Phone: (780) 492-2386. Fax: (780) 492-8916. E-mail: mstiles{at}afns.ualberta.ca.

Applied and Environmental Microbiology, October 1999, p. 4329-4333, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Garneau, S., Ference, C. A., van Belkum, M. J., Stiles, M. E., Vederas, J. C. (2003). Purification and Characterization of Brochocin A and Brochocin B(10-43), a Functional Fragment Generated by Heterologous Expression in Carnobacterium piscicola. Appl. Environ. Microbiol. 69: 1352-1358 [Abstract] [Full Text]  
  • Gänzle, M. G., Höltzel, A., Walter, J., Jung, G., Hammes, W. P. (2000). Characterization of Reutericyclin Produced by Lactobacillus reuteri LTH2584. Appl. Environ. Microbiol. 66: 4325-4333 [Abstract] [Full Text]  


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