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Applied and Environmental Microbiology, October 1999, p. 4340-4345, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Two Cellobiohydrolase-Encoding Genes from Aspergillus niger Require D-Xylose and the Xylanolytic Transcriptional Activator XlnR for Their Expression

Marco M. C. Gielkens, Ester Dekkers, Jaap Visser, and Leo H. de Graaff*

Section Molecular Genetics of Industrial Microorganisms, Wageningen Agricultural University, NL-6703 HA Wageningen, The Netherlands

Received 21 April 1999/Accepted 30 July 1999

Two cellobiohydrolase-encoding genes, cbhA and cbhB, have been isolated from the filamentous fungus Aspergillus niger. The deduced amino acid sequence shows that CbhB has a modular structure consisting of a fungus-type cellulose-binding domain (CBD) and a catalytic domain separated by a Pro/Ser/Thr-rich linker peptide. CbhA consists only of a catalytic domain and lacks a CBD and linker peptide. Both proteins are homologous to fungal cellobiohydrolases in family 7 of the glycosyl hydrolases. Northern blot analysis showed that the transcription of the cbhA and cbhB genes is induced by D-xylose but not by sophorose and, in addition, requires the xylanolytic transcriptional activator XlnR.

* Corresponding author. Mailing address: Section Molecular Genetics of Industrial Microorganisms, Wageningen Agricultural University, Dreijenlaan 2, NL-6703 HA Wageningen, The Netherlands. Phone: 31 317 484691. Fax: 31 317 484011. E-mail: leo.degraaff{at}algemeen.mgim.wau.nl.

Applied and Environmental Microbiology, October 1999, p. 4340-4345, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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