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Applied and Environmental Microbiology, October 1999, p. 4346-4350, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cloning and Analysis of the L-Lactate Utilization Genes from Streptococcus iniae

A. Gibello,1,dagger M. D. Collins,2 L. Domínguez,3,* J. F. Fernández-Garayzábal,3 and P. T. Richardson1

Department of Microbiology, Institute of Food Research, Reading Laboratory, Reading RG6 6BZ,1 and Department of Food Science and Technology, University of Reading, Reading RG6 6AP,2 United Kingdom, and Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain3

Received 2 April 1999/Accepted 27 July 1999

The presence of lactate oxidase was examined in eight Streptococcus species and some related species of bacteria. A clone (pGR002) was isolated from a genomic library of Streptococcus iniae generated in Escherichia coli, containing a DNA fragment spanning two genes designated lctO and lctP. We show that these genes are likely to be involved in the L-lactic acid aerobic metabolism of this organism. This DNA fragment has been sequenced and characterized. A comparison of the deduced amino acid sequence of LctP protein demonstrated that the protein had significant homology with the L-lactate permeases of other bacteria. The amino acid sequence of the LctO protein of S. iniae also showed a strong homology to L-lactate oxidase from Aerococcus viridans and some NAD-independent lactate dehydrogenases, all belonging to the family of flavin mononucleotide-dependent alpha -hydroxyacid-oxidizing enzymes. Biochemical assays of the gene products confirm the identity of the genes from the isolated DNA fragment and reveal a possible role for the lactate oxidase from S. iniae. This lactate oxidase is discussed in relation to the growth of the organism in response to carbon source availability.

* Corresponding author. Mailing address: Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, Avda. Puerta de Hierro s.n., 28040 Madrid, Spain. Phone: 34 91 3943719. Fax: 34 91 3943908. E-mail: Gibelloa{at}eucmax.sim.ucm.es.

dagger Present address: Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.

Applied and Environmental Microbiology, October 1999, p. 4346-4350, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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