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Applied and Environmental Microbiology, October 1999, p. 4425-4430, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of Enterococcus spp. with a Biochemical Key

Albert Manero* and Anicet R. Blanch

Departament de Microbiologia, Universitat de Barcelona, E-08028 Barcelona, Catalonia, Spain

Received 10 February 1999/Accepted 10 July 1999

A six-step biochemical key is presented for the identification of all recognized Enterococcus spp. The key consists of 12 tests, but no more than 6 are needed for the most complicated identification. The reliability of the key has been evaluated with collection type strains and clinical and environmental isolates. This key has fewer tests than those reported in previous studies. There is no commercial kit that includes the whole set of tests. However, some of the tests are included in enzyme activity-based kits that could be used with the proposed key. The key is designed for use in routine applications, especially in environmental and clinical studies with a high number of isolates.

* Corresponding author. Mailing address: Departament de Microbiologia, Universitat de Barcelona, Diagonal 645, E-08028 Barcelona, Catalonia, Spain. Phone: 34 93 4021489. Fax: 34 93 4110592. E-mail: amanero{at}porthos.bio.ub.es.

Applied and Environmental Microbiology, October 1999, p. 4425-4430, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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