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Applied and Environmental Microbiology, October 1999, p. 4458-4463, Vol. 65, No. 10
Centro de Investigaciones Biológicas,
Consejo Superior de Investigaciones Científicas, E-28006
Madrid, Spain
Received 26 April 1999/Accepted 30 July 1999
A versatile peroxidase able to oxidize Mn2+ as well as
phenolic and nonphenolic aromatic compounds is produced in
peptone-containing liquid cultures of Pleurotus eryngii
encoded by the gene mnpl. The regulation of its transcript
levels was investigated by Northern blotting of total RNA.
High-peroxidase transcripts and activity were found in cultures grown
in glucose-peptone medium, whereas only basal levels were detected in
glucose-ammonium medium. The addition of more than 25 µM
Mn2+ to the former medium did not result in detectable
peroxidase transcripts or activity. Potential regulators were also
added to isolated mycelium. In this way, it was shown that high
transcript levels (in peroxidase-expressing mycelium) were maintained
on peptone, whereas expression was not induced in short-term incubation experiments. Similar results were obtained with Mn2+ ions.
Strong induction of mnpl expression was caused by exogenous H2O2 or by continuous
H2O2 generation during redox cycling of menadione. By the use of the latter system in the presence of Fe3+, which catalyzes the reduction of
H2O2 to hydroxyl radical, it was shown for the
first time that the presence of this strong oxidant causes a rapid
increase of the transcripts of a ligninolytic peroxidase. In
conclusion, peptone and Mn2+ affect the levels of
transcripts of this versatile peroxidase in culture, and reduced oxygen
species induce short-term expression in isolated mycelium, probably via
a stress response mechanism.
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Regulation of Peroxidase Transcript Levels in
Liquid Cultures of the Ligninolytic Fungus Pleurotus
eryngii
*
Corresponding author. Mailing address: CIB, CSIC,
Velázquez 144, E-28006 Madrid, Spain. Phone: 34 915611800. Fax:
34 915627518. E-mail: ATMartinez{at}cib.csic.es.
Applied and Environmental Microbiology, October 1999, p. 4458-4463, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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