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Applied and Environmental Microbiology, October 1999, p. 4470-4474, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Production and Characterization of Monoclonal Antibodies against the Hemolysin BL Enterotoxin Complex Produced by Bacillus cereus

R. Dietrich, C. Fella, S. Strich, and E. Märtlbauer*

Institute for Hygiene and Technology of Food of Animal Origin, Veterinary Faculty, University of Munich, 80539 Munich, Germany

Received 19 April 1999/Accepted 10 July 1999

A total of five hybridoma cell lines that produced monoclonal antibodies against the components of the hemolysin BL (HBL) enterotoxin complex and sphingomyelinase produced by Bacillus cereus were established and characterized. Monoclonal antibody 2A3 was specific for the B component, antibodies 1A12 and 8B12 were specific for the L2 component, and antibody 1C2 was specific for the L1 protein of the HBL enterotoxin complex. No cross-reactivity with other proteins produced by different strains of B. cereus was observed for monoclonal antibodies 2A3, 1A12, and 8B12, whereas antibody 1C2 cross-reacted with an uncharacterized protein of approximately 93 kDa and with a 39-kDa protein, which possibly represents one component of the nonhemolytic enterotoxin complex. Antibody 2A12 finally showed a distinct reactivity with B. cereus sphingomyelinase. The monoclonal antibodies developed in this study were also successfully applied in indirect enzyme immunoassays for the characterization of the enterotoxic activity of B. cereus strains. About 50% of the strains tested were capable of producing the HBL enterotoxin complex, and it could be demonstrated that all strains producing HBL were also highly cytotoxic.

* Corresponding author. Mailing address: Institut für Hygiene und Technologie der Lebensmittel, Tiermedizinische Fakultät, Universität München, Veterinärstrasse 13, 80539 München, Germany. Phone: 49 89 2180 3672. Fax: 49 89 2180 2106. E-mail: E.Maertlbauer{at}mh.vetmed.uni-muenchen.de.

Applied and Environmental Microbiology, October 1999, p. 4470-4474, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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