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Applied and Environmental Microbiology, October 1999, p. 4672-4676, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Helicobacter Species Colonizing Pig Stomach: Molecular Characterization and Determination of Prevalence

Franck Cantet,1 Catherine Magras,2 Armelle Marais,1 Michel Federighi,2 and Francis Mégraud1,*

Laboratoire de Bactériologie, Hôpital Pellegrin, and Université Victor Ségalen Bordeaux 2, Bordeaux,1 and Unité mixte de recherche d'Hygiène Alimentaire INRA/Ecole Nationale Vétérinaire, Nantes,2 France.

Received 11 May 1999/Accepted 3 August 1999

The infection rate of 60 pigs (10 pigs from each of six farms) by Helicobacter species was studied by two techniques. Histological examination of the cardiac area of the stomach yielded a 58% positive result versus an 80% positive result by PCR with genus-specific primers. Eighty percent of the 16S rRNA gene was amplified, classified in four groups by PCR-restriction fragment length polymorphism, and sequenced. Isolates from all farms except one (farm C) were identified as Helicobacter heilmannii type 1, while those from farm C were identified as H. heilmannii type 2. Attempts to culture this organism in vitro failed. Helicobacter pylori was not found in these animals.

* Corresponding author. Mailing address: Laboratoire de Bactériologie, Hôpital Pellegrin, Place Amélie Raba-Léon, 33076 Bordeaux Cedex, France. Phone: (33) 5 56 79 59 10. Fax: (33) 5 56 79 60 18. E-mail: francis.megraud{at}chu-aquitaine.fr.

Applied and Environmental Microbiology, October 1999, p. 4672-4676, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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