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Applied and Environmental Microbiology, October 1999, p. 4685-4687, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Bacterial Production of D-Erythroascorbic Acid and L-Ascorbic Acid through Functional Expression of Saccharomyces cerevisiae D-Arabinono-1,4-Lactone Oxidase in Escherichia coli

Byung-Hoon Lee, Won-Ki Huh, Seong-Tae Kim,dagger Jung-Shin Lee, and Sa-Ouk Kang*

Laboratory of Biophysics, Department of Microbiology, College of Natural Sciences, and Research Center for Molecular Microbiology, Seoul National University, Seoul 151-742, Republic of Korea

Received 21 May 1999/Accepted 2 August 1999

D-Arabinono-1,4-lactone oxidase, which catalyzes the terminal step in the biosynthesis of D-erythroascorbic acid in Saccharomyces cerevisiae, was functionally expressed in Escherichia coli inherently lacking the enzyme. The recombinant E. coli strain expressing the enzyme could overproduce D-erythroascorbic acid and L-ascorbic acid when supplied with D-arabinono-1,4-lactone and L-galactono-1,4-lactone, respectively.

* Corresponding author. Mailing address: Laboratory of Biophysics, Department of Microbiology, College of Natural Sciences, and Research Center for Molecular Microbiology, Seoul National University, Seoul 151-742, Republic of Korea. Phone: 82-2-880-6703. Fax: 82-2-888-4911. E-mail: kangsaou{at}plaza.snu.ac.kr.

dagger Present address: Department of Hematology-Oncology, St. Jude Children's Research Hospital, Memphis, TN 38105-2794.

Applied and Environmental Microbiology, October 1999, p. 4685-4687, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sauer, M., Branduardi, P., Valli, M., Porro, D. (2004). Production of L-Ascorbic Acid by Metabolically Engineered Saccharomyces cerevisiae and Zygosaccharomyces bailii. Appl. Environ. Microbiol. 70: 6086-6091 [Abstract] [Full Text]  
  • Salusjarvi, T., Kalkkinen, N., Miasnikov, A. N. (2004). Cloning and Characterization of Gluconolactone Oxidase of Penicillium cyaneo-fulvum ATCC 10431 and Evaluation of Its Use for Production of D-Erythorbic Acid in Recombinant Pichia pastoris. Appl. Environ. Microbiol. 70: 5503-5510 [Abstract] [Full Text]  
  • Huh, W.-K., Kim, S.-T., Kim, H., Jeong, G., Kang, S.-O. (2001). Deficiency of D-Erythroascorbic Acid Attenuates Hyphal Growth and Virulence of Candida albicans. Infect. Immun. 69: 3939-3946 [Abstract] [Full Text]  


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