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Applied and Environmental Microbiology, October 1999, p. 4688-4692, Vol. 65, No. 10
Lehrstuhl für Mikrobiologie,
Received 8 March 1999/Accepted 23 June 1999
The iap gene encodes the protein p60, which is common
to all Listeria species. A previous comparison of the DNA
sequences indicated conserved and species-specific gene portions. Based on these comparisons, a combination consisting of only five different primers that allows the specific detection and differentiation of
Listeria species with a single multiplex PCR and subsequent gel analysis was selected. One primer was derived from the conserved 3'
end and is specific for all Listeria species; the other
four primers are specific for Listeria monocytogenes,
L. innocua, L. grayi, or the three grouped
species L. ivanovii, L. seeligeri, and L. welshimeri, respectively. The PCR method, which also enables the
simultaneous detection of L. monocytogenes and L. innocua, was evaluated against conventional biotyping with 200 food hygiene-relevant Listeria strains. The results
indicated the superiority of this technique. Thus, this novel type of
multiplex PCR may be useful for rapid Listeria species
confirmation and for identification of Listeria species for
strains isolated from different sources.
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Detection and Differentiation of
Listeria spp. by a Single Reaction Based on Multiplex
PCR
*
Corresponding author. Mailing address: Merck KGaA,
Scientific Laboratory Products, Microbiological Analytics, Frankfurter Strasse 250, 64293 Darmstadt, Germany. Phone: 49-6151-727661. Fax:
49-6151-726904. E-mail: andreas.bubert{at}merck.de.
Applied and Environmental Microbiology, October 1999, p. 4688-4692, Vol. 65, No. 10
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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